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Facteurs de Croissance et Cytokines

Recombinant Human Flt3-Ligand

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Product Details

Catalogue Number: 300-19
Description:
Recombinant Human Flt3-Ligand

Flt3-Ligand is a growth factor that regulates proliferation of early hematopoietic cells. Flt3-Ligand binds to cells expressing the tyrosine kinase receptor Flt3. Flt3-Ligand, by itself does not stimulate proliferation of early hematopoietic cells, but synergizes with other CSFs and interleukins to induce growth and differentiation. Unlike SCF, Flt3-Ligand exerts no activity on mast cells. Multiple isoforms of Flt3-Ligand have been identified. The predominant biologically active form is anchored to the cell surface as the extracellular domain of a transmembrane protein (209 a.a.). The membrane-bound isoform can be proteolytically cleaved to generate a biologically active soluble isoform. Recombinant Human Flt3-Ligand is a soluble 17.6 kDa protein consisting of 155 amino acid residues.

Source: E.coli

Synonyms: Fms-related tyrosine kinase 3 ligand, Flt3L

AA Sequence: TQDCSFQHSP ISSDFAVKIR ELSDYLLQDY PVTVASNLQD EELCGGLWRL VLAQRWMERL KTVAGSKMQG LLERVNTEIH FVTKCAFQPP PSCLRFVQTN ISRLLQETSE QLVALKPWIT RQNFSRCLEL QCQPDSSTLP PPWSPRPLEA TAPTA

Purity: ≥ 98% by SDS-PAGE gel and HPLC analyses.

Biological Activity: The ED50 was determined by the dose-dependent stimulation of the proliferation of human AML5 cells is ≤ 1.0 ng/ml, corresponding to a specific activity of ≥ 1 x 106 units/mg.

This product has been tested against the WHO standard. International Unit information can be found here.

Recombinant Human Flt3-Ligand WHO Comparison Graph Recombinant Human Flt3-Ligand Biological Activity Graph Recombinant Human Flt3-Ligand WHO Comparison Graph Recombinant Human Flt3-Ligand Biological Activity Graph

Calculated Molecular Weight: 17.6 kDa

Accession Number: P49771

Gene ID: 2322

Endotoxin: Endotoxin level is < 0.1 ng/ug of protein (< 1 EU/ug)

crossreactivity:
Country Of Origin: USA

Not for human use.

Research Interest

product.subtitle.recentcitations

First Author
Li, Y
Title
Modulation of WNT, Activin/Nodal, and MAPK Signaling Pathways Increases Arterial Hemogenic Endothelium and Hematopoietic Stem/Progenitor Cell Formation During Human iPSC Differentiation.
Citation
Stem cells (Dayton, Ohio); 41(7) pg685-697
PubMed Id
First Author
Thomas, G E
Title
The metabolic enzyme hexokinase 2 localizes to the nucleus in AML and normal haematopoietic stem and progenitor cells to maintain stemness.
Citation
Nature Cell Biology; 24(6) pg872-884
PubMed Id
First Author
Tvorogov, D
Title
Targeting human CALR-mutated MPN progenitors with a neoepitope-directed monoclonal antibody.
Citation
EMBO reports; 23(4) pge52904
PubMed Id