Recombinant Human Flt3-Ligand 0 ReviewsSubmit a Review Product Details Catalogue Number: 300-19 Description: Flt3-Ligand is a growth factor that regulates proliferation of early hematopoietic cells. Flt3-Ligand binds to cells expressing the tyrosine kinase receptor Flt3. Flt3-Ligand, by itself does not stimulate proliferation of early hematopoietic cells, but synergizes with other CSFs and interleukins to induce growth and differentiation. Unlike SCF, Flt3-Ligand exerts no activity on mast cells. Multiple isoforms of Flt3-Ligand have been identified. The predominant biologically active form is anchored to the cell surface as the extracellular domain of a transmembrane protein (209 a.a.). The membrane-bound isoform can be proteolytically cleaved to generate a biologically active soluble isoform. Recombinant Human Flt3-Ligand is a soluble 17.6 kDa protein consisting of 155 amino acid residues. Source: E.coli Synonyms: Fms-related tyrosine kinase 3 ligand, Flt3L AA Sequence: TQDCSFQHSP ISSDFAVKIR ELSDYLLQDY PVTVASNLQD EELCGGLWRL VLAQRWMERL KTVAGSKMQG LLERVNTEIH FVTKCAFQPP PSCLRFVQTN ISRLLQETSE QLVALKPWIT RQNFSRCLEL QCQPDSSTLP PPWSPRPLEA TAPTA Purity: ≥ 98% by SDS-PAGE gel and HPLC analyses. Biological Activity: The ED50 was determined by the dose-dependent stimulation of the proliferation of human AML5 cells is ≤ 1.0 ng/ml, corresponding to a specific activity of ≥ 1 x 106 units/mg. This product has been tested against the WHO standard. International Unit information can be found here. Calculated Molecular Weight: 17.6 kDa Accession Number: P49771 Gene ID: 2322 Endotoxin: Endotoxin level is < 0.1 ng/ug of protein (< 1 EU/ug) crossreactivity: Human, Human + Hamster, Human + Monkey, Human + Mouse, Monkey, Mouse, Rabbit, Rat References PubMed SDS CoA Search Product Line Country Of Origin: USA Not for human use. Research Interest Cancer Cell Culture Immune System Stem Cells & Differentiation product.subtitle.recentcitations First Author Li, Y Title Modulation of WNT, Activin/Nodal, and MAPK Signaling Pathways Increases Arterial Hemogenic Endothelium and Hematopoietic Stem/Progenitor Cell Formation During Human iPSC Differentiation. Citation Stem cells (Dayton, Ohio); 41(7) pg685-697 PubMed Id 37220178 First Author Thomas, G E Title The metabolic enzyme hexokinase 2 localizes to the nucleus in AML and normal haematopoietic stem and progenitor cells to maintain stemness. Citation Nature Cell Biology; 24(6) pg872-884 PubMed Id 35668135 First Author Tvorogov, D Title Targeting human CALR-mutated MPN progenitors with a neoepitope-directed monoclonal antibody. Citation EMBO reports; 23(4) pge52904 PubMed Id 35156745
