Facteurs de Croissance et Cytokines

Recombinant Human HGF (Insect derived)

Product Details

Catalogue Number: 100-39
Description:

HGF is a potent, mesenchymally-derived mitogen for mature parenchymal hepatocytes, and acts as a growth factor for a broad spectrum of tissues and cell types. HGF signals through a transmembrane tyrosine kinase receptor known as MET. Activities of HGF include the induction of cell proliferation, motility, morphogenesis, inhibition of cell growth, and enhancement of neuron survival. HGF is a crucial mitogen for liver regeneration processes, especially after partial hepatectomy and other liver injuries. Human and murine HGF are cross-reactive. Human HGF is expressed as a linear, polypeptide-precursor glycoprotein containing 697 amino acid residues. Proteolytic processing of this precursor generates the biologically active heterodimeric form of HGF, which consists of two polypeptide chains (α-chain and β-chain) held together by a single disulfide bond resulting in formation of a biologically active heterodimer. The α-chain consists of 463 amino acid residues and four kringle domains. The β-chain consists of 234 amino acid residues. Recombinant Human HGF is an 80.0 kDa polypeptide consisting of 697 amino acid residues.

Source: (BTI-Tn-5B1-4) Hi-5 Insect cells

Synonyms: Hepatocyte Growth Factor, Scatter Factor (SF), Hepatopoietin (HPTA)

AA Sequence:

alpha chain: QRKRRNTIHE FKKSAKTTLI KIDPALKIKT KKVNTADQCA NRCTRNKGLP FTCKAFVFDK ARKQCLWFPF NSMSSGVKKE FGHEFDLYEN KDYIRNCIIG KGRSYKGTVS ITKSGIKCQP WSSMIPHEHS FLPSSYRGKD LQENYCRNPR GEEGGPWCFT SNPEVRYEVC DIPQCSEVEC MTCNGESYRG LMDHTESGKI CQRWDHQTPH RHKFLPERYP DKGFDDNYCR NPDGQPRPWC YTLDPHTRWE YCAIKTCADN TMNDTDVPLE TTECIQGQGE GYRGTVNTIW NGIPCQRWDS QYPHEHDMTP ENFKCKDLRE NYCRNPDGSE SPWCFTTDPN IRVGYCSQIP NCDMSHGQDC YRGNGKNYMG NLSQTRSGLT CSMWDKNMED LHRHIFWEPD ASKLNENYCR NPDDDAHGPW CYTGNPLIPW DYCPISRCEG DTTPTIVNLD HPVISCAKTK QLR

beta chain: VVNGIP TRTNIGWMVS LRYRNKHICG GSLIKESWVL TARQCFPSRD LKDYEAWLGI HDVHGRGDEK CKQVLNVSQL VYGPEGSDLV LMKLARPAVL DDFVSTIDLP NYGCTIPEKT SCSVYGWGYT GLINYDGLLR VAHLYIMGNE KCSQHHRGKV TLNESEICAG AEKIGSGPCE GDYGGPLVCE QHKMRMVLGV IVPGRGCAIP NRPGIFVRVA YYAKWIHKII LTYKVPQS

Purity: ≥ 98% by SDS-PAGE gel and HPLC analyses.

Biological Activity: Determined by the dose-dependent stimulation of the proliferation of monkey 4MBr-5 cells. The expected ED50 for this effect is 1.0-20.0 ng/ml.

This product has been tested against the WHO standard. International Unit information can be found here.

Calculated Molecular Weight: 80 kDa

Accession Number: P14210

Gene ID: 3082

Note:

250μg is the largest vial size for this product. Larger sizes supplied as multiple 250μg vials.

crossreactivity:
Country Of Origin: USA

Not for human use.

Research Interest

product.subtitle.recentcitations

First Author
Fujiwara, M
Title
Parkin as a tumor suppressor gene for hepatocellular carcinoma.
Citation
Oncogene; 27(46) pg6002-11
PubMed Id
First Author
AlSudais, H
Title
CCAAT/Enhancer Binding Protein β inhibits myogenic differentiation via ID3.
Citation
Scientific Reports; 8(1) pg16613
PubMed Id
First Author
Usatyuk, P V
Title
Role of c-Met/phosphatidylinositol 3-kinase (PI3k)/Akt signaling in hepatocyte growth factor (HGF)-mediated lamellipodia formation, reactive oxygen species (ROS) generation, and motility of lung endothelial cells.
Citation
The Journal of Biological Chemistry; 289(19) pg13476-91
PubMed Id

product.subtitle.productreviews

Existing reviews
Good quality product and excellent service
I have had great results and excellent service from Peprotech. hHGF (insect derived) always worked as expected and consistently for developing liver oragnoids.
From:  Afshan | Date:  28/07/2020
Hepatocyte Growth Factor for Liver Cancer Organoid Culture
Using HGF, Able to culture the Hepatobilliary Cancer Organoids.
Quick Next day delivery is great.
From:  Hardik | Date:  31/08/2020