Facteurs de Croissance et Cytokines

Recombinant Human HGF (Insect derived)

Product Details

Catalogue Number: 100-39
Description:
Recombinant Human HGF (Insect derived)

HGF is a potent, mesenchymally-derived mitogen for mature parenchymal hepatocytes, and acts as a growth factor for a broad spectrum of tissues and cell types. HGF signals through a transmembrane tyrosine kinase receptor known as MET. Activities of HGF include the induction of cell proliferation, motility, morphogenesis, inhibition of cell growth, and enhancement of neuron survival. HGF is a crucial mitogen for liver regeneration processes, especially after partial hepatectomy and other liver injuries. Human and murine HGF are cross-reactive. Human HGF is expressed as a linear, polypeptide-precursor glycoprotein containing 697 amino acid residues. Proteolytic processing of this precursor generates the biologically active heterodimeric form of HGF, which consists of two polypeptide chains (α-chain and β-chain) held together by a single disulfide bond resulting in formation of a biologically active heterodimer. The α-chain consists of 463 amino acid residues and four kringle domains. The β-chain consists of 234 amino acid residues. Recombinant Human HGF is an 80.0 kDa polypeptide consisting of 697 amino acid residues.

Source: (BTI-Tn-5B1-4) Hi-5 Insect cells

Synonyms: Hepatocyte Growth Factor, Scatter Factor (SF), Hepatopoietin (HPTA)

AA Sequence:

alpha chain: QRKRRNTIHE FKKSAKTTLI KIDPALKIKT KKVNTADQCA NRCTRNKGLP FTCKAFVFDK ARKQCLWFPF NSMSSGVKKE FGHEFDLYEN KDYIRNCIIG KGRSYKGTVS ITKSGIKCQP WSSMIPHEHS FLPSSYRGKD LQENYCRNPR GEEGGPWCFT SNPEVRYEVC DIPQCSEVEC MTCNGESYRG LMDHTESGKI CQRWDHQTPH RHKFLPERYP DKGFDDNYCR NPDGQPRPWC YTLDPHTRWE YCAIKTCADN TMNDTDVPLE TTECIQGQGE GYRGTVNTIW NGIPCQRWDS QYPHEHDMTP ENFKCKDLRE NYCRNPDGSE SPWCFTTDPN IRVGYCSQIP NCDMSHGQDC YRGNGKNYMG NLSQTRSGLT CSMWDKNMED LHRHIFWEPD ASKLNENYCR NPDDDAHGPW CYTGNPLIPW DYCPISRCEG DTTPTIVNLD HPVISCAKTK QLR

beta chain: VVNGIP TRTNIGWMVS LRYRNKHICG GSLIKESWVL TARQCFPSRD LKDYEAWLGI HDVHGRGDEK CKQVLNVSQL VYGPEGSDLV LMKLARPAVL DDFVSTIDLP NYGCTIPEKT SCSVYGWGYT GLINYDGLLR VAHLYIMGNE KCSQHHRGKV TLNESEICAG AEKIGSGPCE GDYGGPLVCE QHKMRMVLGV IVPGRGCAIP NRPGIFVRVA YYAKWIHKII LTYKVPQS

Purity: ≥ 98% by SDS-PAGE gel and HPLC analyses.

Biological Activity: Determined by the dose-dependent stimulation of the proliferation of monkey 4MBr-5 cells. The expected ED50 for this effect is 1.0-20.0 ng/ml.

This product has been tested against the WHO standard. International Unit information can be found here.

Recombinant Human HGF (Insect derived) Biological Activity Graph Recombinant Human HGF (Insect derived) WHO Comparison Biological Activity Graph

Calculated Molecular Weight: 80 kDa

Accession Number: P14210

Gene ID: 3082

Note:

250μg is the largest vial size for this product. Larger sizes supplied as multiple 250μg vials.

crossreactivity:
Country Of Origin: USA

Not for human use.

Research Interest

product.subtitle.recentcitations

First Author
Kawase, N
Title
SRC kinase activator CDCP1 promotes hepatocyte growth factor-induced cell migration/invasion of a subset of breast cancer cells.
Citation
The Journal of Biological Chemistry; 298(3) pg101630
PubMed Id
First Author
Belenguer, G
Title
RNF43/ZNRF3 loss predisposes to hepatocellular-carcinoma by impairing liver regeneration and altering the liver lipid metabolic ground-state.
Citation
Nature Communications; 13(1) pg334
PubMed Id
First Author
Yun, W
Title
OCT4-induced oligodendrocyte progenitor cells promote remyelination and ameliorate disease.
Citation
NPJ Regenerative Medicine; 7(1) pg4
PubMed Id

product.subtitle.productreviews

Existing reviews
Good quality product and excellent service
I have had great results and excellent service from Peprotech. hHGF (insect derived) always worked as expected and consistently for developing liver oragnoids.
From:  Afshan | Date:  28/07/2020
Hepatocyte Growth Factor for Liver Cancer Organoid Culture
Using HGF, Able to culture the Hepatobilliary Cancer Organoids.
Quick Next day delivery is great.
From:  Hardik | Date:  31/08/2020