Facteurs de Croissance et Cytokines

Recombinant Human IL-2

Product Details

Catalogue Number: 200-02
Description:

IL-2 is a powerful immunoregulatory lymphokine produced by T cells in response to antigenic or mitogenic stimulation. IL-2/IL-2R signaling is required for T cell proliferation and other fundamental functions that are essential for the immune response. IL-2 stimulates growth and differentiation of B cells, NK cells, lymphokine-activated killer cells, monocytes, macrophages and oligodendrocytes. Recombinant Human IL-2 is a 15.5 kDa protein, containing 134 amino acid residues including one intrachain disulfide bond.

Source: E.coli

Synonyms: Interleukin-2, T-cell growth factor (TCGF), Aldesleukin

AA Sequence: MAPTSSSTKK TQLQLEHLLL DLQMILNGIN NYKNPKLTRM LTFKFYMPKK ATELKHLQCL EEELKPLEEV LNLAQSKNFH LRPRDLISNI NVIVLELKGS ETTFMCEYAD ETATIVEFLN RWITFAQSII STLT

Purity: ≥ 98% by SDS-PAGE gel and HPLC analyses.

Biological Activity: The ED50 as determined by the dose-dependent stimulation of murine CTLL-2 cells is ≤ 0.1 ng/ml, corresponding to a specific activity of ≥ 1 x 107 units/mg.

This product has been tested against the WHO standard. International Unit information can be found here.

Calculated Molecular Weight: 15.5 kDa

Accession Number: P60568

Gene ID: 3558

crossreactivity:
Country Of Origin: USA

Not for human use.

Research Interest

product.subtitle.recentcitations

First Author
Saverino, D
Title
CTLA-4 (CD152) inhibits the specific lysis mediated by human cytolytic T lymphocytes in a clonally distributed fashion.
Citation
Journal of immunology (Baltimore, Md. : 1950); 162(2) pg651-8
PubMed Id
First Author
Imada, K
Title
Stat5b is essential for natural killer cell-mediated proliferation and cytolytic activity.
Citation
The Journal of Experimental Medicine; 188(11) pg2067-74
PubMed Id
First Author
Thornton, A M
Title
CD4+CD25+ immunoregulatory T cells suppress polyclonal T cell activation in vitro by inhibiting interleukin 2 production.
Citation
The Journal of Experimental Medicine; 188(2) pg287-96
PubMed Id

product.subtitle.productreviews

Existing reviews
Excellent for Treg culture
Very good for culture of human sorted Tregs, consistent across lots and vials.
From:  Julian | Date:  10/07/2020
hIL-2
Great product, works well to stimulate primary human T cells (tested for up to 2 weeks in culture).
From:  Kimberly | Date:  10/08/2020
T cell culture
Used in culturing primary human T cells - works great!
From:  Elizabeth | Date:  26/08/2020
hIL2
Very good product for culturing T cells in vitro. Works great.
From:  Kevin | Date:  26/08/2020
Primary T cells
Worked well in expanding activated primary T cells. Customer service was great when I wanted to know its equivalent IU values.
From:  Adam | Date:  27/08/2020
Works just fine
Aliquoted as recommended and worked just fine for several months.
From:  Tanja | Date:  27/08/2020