Facteurs de Croissance et Cytokines

Recombinant Human NOV

Product Details

Catalogue Number: 120-26
Description:

NOV is a member of the CCN family of secreted, cysteine-rich regulatory proteins. The full-length NOV protein contains four structural domains that confer distinct, and sometimes opposing, biological activities. Elevated expression of NOV is associated with certain tumors, including Wilm’s tumor and most nephroblastomas.  However, in other tumor types and certain cancer cell lines, increased tumorigenicity and proliferation is correlated with decreased NOV expression. Additionally, NOV induces cell adhesion and cell migration by signaling through specific cell-surface integrins, and by binding to heparin sulfate proteoglycans and to fibulin 1C. NOV has also been reported to exert proangiogenic activities.  Recombinant Human NOV is a 36.2 kDa protein containing 331 amino acid residues. It is composed of four distinct structural domains (modules): the IGF binding protein (IGFBP) domain; the von Willebrand Factor C (VWFC) domain; the Thrombospondin type-I (TSP type-1) domain; and a C-terminal cysteine knot-like domain (CTCK).

Source: E.coli

Synonyms: Nephroblastoma Overexpressed gene, CCN3, IGFBP9, NovH

AA Sequence: MQVAATQRCP PQCPGRCPAT PPTCAPGVRA VLDGCSCCLV CARQRGESCS DLEPCDESSG LYCDRSADPS NQTGICTAVE GDNCVFDGVI YRSGEKFQPS CKFQCTCRDG QIGCVPRCQL DVLLPEPNCP APRKVEVPGE CCEKWICGPD EEDSLGGLTL AAYRPEATLG VEVSDSSVNC IEQTTEWTAC SKSCGMGFST RVTNRNRQCE MLKQTRLCMV RPCEQEPEQP TDKKGKKCLR TKKSLKAIHL QFKNCTSLHT YKPRFCGVCS DGRCCTPHNT KTIQAEFQCS PGQIVKKPVM VIGTCTCHTN CPKNNEAFLQ ELELKTTRGK M

Purity: ≥ 95% by SDS-PAGE gel and HPLC analyses.

Biological Activity: Determined by a cell proliferation assay using BALB/c 3T3 cells. The expected ED50 for this effect is 1.0-2.0 μg/ml

Calculated Molecular Weight: 36.2 kDa

Accession Number: P48745

Gene ID: 4856

crossreactivity:
Country Of Origin: USA

Not for human use.

Research Interest

product.subtitle.recentcitations

First Author
Lorda-Diez, C I
Title
Defining the earliest transcriptional steps of chondrogenic progenitor specification during the formation of the digits in the embryonic limb.
Citation
PLoS ONE; 6(9) pge24546
PubMed Id
First Author
Rydziel, S
Title
Nephroblastoma overexpressed (Nov) inhibits osteoblastogenesis and causes osteopenia.
Citation
The Journal of Biological Chemistry; 282(27) pg19762-72
PubMed Id
First Author
McCallum, L
Title
A novel mechanism for BCR-ABL action: stimulated secretion of CCN3 is involved in growth and differentiation regulation.
Citation
Blood; 108(5) pg1716-23
PubMed Id