Recombinant Human sCD40 Ligand 0 ReviewsSubmit a Review Product Details Catalogue Number: 310-02 Description: CD40, a member of the TNF receptor family, is a cell surface protein expressed on B cells, dendritic cells, monocytes, thymic epithelial cells and, at low levels, on T cells. Signaling though CD40 plays an important role in the proliferation and differentiation of B cells, and is critical for immunoglobulin (Ig) class switching. The membrane-anchored CD40 Ligand is expressed almost exclusively on activated CD4+ T lymphocytes. Failure to express CD40L leads to "immunodeficiency with hyper-IgM", a disease characterized by failure to produce IgG, IgA and IgE. The human CD40L gene codes for a 261 amino acid type II transmembrane protein, which contains a 22 amino acid cytoplasmic domain, a 24 amino acid transmembrane domain, and a 215 amino acid extracellular domain. The soluble form of CD40L is an 18 kDa protein comprising the entire TNF homologous region of CD40L and is generated in vivo by an intracellular proteolytic processing of the full length CD40L. Recombinant Human soluble CD40 ligand is a 16.3 kDa protein containing 149 amino acid residues comprising the receptor binding TNF-like domain of CD40L. Source: E.coli Synonyms: soluble CD40 Ligand, TNFSF5, TRAP, CD154, Gp39, T-BAM AA Sequence: MQKGDQNPQI AAHVISEASS KTTSVLQWAE KGYYTMSNNL VTLENGKQLT VKRQGLYYIY AQVTFCSNRE ASSQAPFIAS LWLKSPGRFE RILLRAANTH SSAKPCGQQS IHLGGVFELQ PGASVFVNVT DPSQVSHGTG FTSFGLLKL Purity: ≥ 98% by SDS-PAGE gel and HPLC analyses. Biological Activity: Determined by its ability to stimulate the proliferation of HEK-BlueTM CD40 reporter cells. Calculated Molecular Weight: 16.3 kDa Accession Number: P29965 Gene ID: 959 Endotoxin: Endotoxin level is < 0.1 ng/ug of protein (< 1 EU/ug) crossreactivity: Bacteria, Human, Monkey, Mouse References PubMed SDS CoA Search Product Line Country Of Origin: USA Not for human use. Research Interest Organoids Angiogenesis/Cardiovascular Apoptosis Immune System Inflammation TNF Superfamily Allergy Transplantation product.subtitle.recentcitations First Author Sharma, S Title miR-29 modulates CD40 signaling in chronic lymphocytic leukemia by targeting TRAF4: an axis affected by BCR inhibitors. Citation Blood; 137(18) pg2481-2494 PubMed Id 33171493 First Author Mandolesi, M Title SARS-CoV-2 protein subunit vaccination of mice and rhesus macaques elicits potent and durable neutralizing antibody responses. Citation Cell Reports. Medicine; 2(4) pg100252 PubMed Id 33842900 First Author Chen, Z Title SDF-1α Facilitates Mesenchymal Stem Cells to Induce Regulatory B Cell Differentiation from Patients with Immune Thrombocytopenia. Citation Stem Cells International; 2021 pg3254488 PubMed Id 34790240