Animal-Free Recombinant Human sCD40 Ligand 0 AvisSubmit a Review Détails du Produit Catalog Number: AF-310-02 Description: CD40, a member of the TNF receptor superfamily, is a cell surface protein expressed on B cells, dendritic cells, monocytes, thymic epithelial cells and, at low levels, on T cells. Signaling though CD40 plays an important role in the proliferation and differentiation of B cells, and is critical for immunoglobulin (Ig) class switching. The membrane-anchored CD40 Ligand is expressed almost exclusively on activated CD4+ T lymphocytes. Failure to express CD40L leads to “immunodeficiency with hyper-IgM”, a disease characterized by failure to produce IgG, IgA and IgE. The human CD40L gene codes for a 261 amino acid type II transmembrane protein, which contains a 22 amino acid cytoplasmic domain, a 24 amino acid transmembrane domain, and a 215 amino acid extracellular domain. The soluble form of CD40L is an 18 kDa protein comprising the entire TNF homologous region of CD40L and is generated in vivo by an intracellular proteolytic processing of the full length CD40L. Recombinant Human soluble CD40 ligand is a 16.3 kDa protein containing 149 amino acid residues comprising the receptor binding TNF-like domain of CD40L. Manufactured using all Animal-Free reagents. Source: E.coli Synonyms: soluble CD40 Ligand, TNFSF5, TRAP, CD154, Gp39, T-BAM AA Sequence: MQKGDQNPQI AAHVISEASS KTTSVLQWAE KGYYTMSNNL VTLENGKQLT VKRQGLYYIY AQVTFCSNRE ASSQAPFIAS LWLKSPGRFE RILLRAANTH SSAKPCGQQS IHLGGVFELQ PGASVFVNVT DPSQVSHGTG FTSFGLLKL Purity: ≥ 98% by SDS-PAGE gel and HPLC analyses. Biological Activity: Determined by its ability to stimulate the proliferation of HEK-BlueTM CD40 reporter cells. Calculated Molecular Weight: 16.3 kDa Accession Number: P29965 Gene ID: 959 Endotoxin: Endotoxin level is < 0.01 ng/ug of protein (< 0.1 EU/ug) crossreactivity: Human References PubMed SDS Recherche de CoA Product Line Country Of Origin: USA Not for human use. Research Interest Organoids Angiogenesis/Cardiovascular Apoptosis Immune System Inflammation TNF Superfamily Allergy Transplantation product.subtitle.recentcitations Premier Auteur Zhang, T Titre An NKp30-based chimeric antigen receptor promotes T cell effector functions and antitumor efficacy in vivo. Citation Journal of immunology (Baltimore, Md. : 1950); 189(5) pg2290-9 Id PubMed 22851709