Animal-Free Recombinant Human SCF 1 ReviewsSubmit a Review Product Details Catalogue Number: AF-300-07 Description: SCF is a hematopoietic growth factor that exerts its activity by signaling through the c-Kit receptor. SCF and c-Kit are essential for the survival, proliferation and differentiation of hematopoietic cells committed to the melanocyte and germ cell lineages. Human SCF manifests low activity on murine cells, while murine and rat SCF are fully active on human cells. The human SCF gene encodes for a 273 amino acid transmembrane protein, which contains a 25 amino acid N-terminal signal sequence, a 189 amino acid extracellular domain, a 23 amino acid transmembrane domain, and a 36 amino acid cytoplasmic domain. The secreted soluble form of SCF is generated by proteolytic processing of the membrane anchored precursor. Recombinant Human SCF is an 18.4 kDa polypeptide containing 165 amino acid residues, which corresponds to the sequence of the secreted soluble form of SCF. Manufactured using all Animal-Free reagents. Source: E.coli Synonyms: Stem Cell Factor, c-Kit Ligand, Mast Cell Growth Factor (MGF), Steel Factor AA Sequence: MEGICRNRVT NNVKDVTKLV ANLPKDYMIT LKYVPGMDVL PSHCWISEMV VQLSDSLTDL LDKFSNISEG LSNYSIIDKL VNIVDDLVEC VKENSSKDLK KSFKSPEPRL FTPEEFFRIF NRSIDAFKDF VVASETSDCV VSSTLSPEKD SRVSVTKPFM LPPVA Purity: ≥ 98% by SDS-PAGE gel and HPLC analyses. Biological Activity: The ED50 was determined by the dose-dependent stimulation of the proliferation of human TF-1 cells is ≤ 2.0 ng/ml, corresponding to a specific activity of ≥ 5 x 105 units/mg. Calculated Molecular Weight: 18.4 kDa Accession Number: P21583 Gene ID: 4254 Endotoxin: Endotoxin level is < 0.01 ng/ug of protein (< 0.1 EU/ug) crossreactivity: Human, Monkey, Mouse, Rabbit References PubMed SDS CoA Search Product Line Country Of Origin: USA Not for human use. Research Interest Cell Culture Immune System Stem Cells & Differentiation product.subtitle.recentcitations First Author King, A J Title Reactivation of a developmentally silenced embryonic globin gene Citation Nature Communications; 12(1) pg4439 PubMed Id 34290235 First Author Giuli, M V Title Notch3 contributes to T-cell leukemia growth via regulation of the unfolded protein response Citation Oncogenesis; 9(10) pg93 PubMed Id 33071287 First Author Thomé, C H Title NTAL is associated with treatment outcome, cell proliferation and differentiation in acute promyelocytic leukemia Citation Scientific Reports; 10(1) pg10315 PubMed Id 32587277 product.subtitle.productreviews Existing reviews For differentiation macrophage progenitors. I have been using this human SCF for differentiating macrophage progenitors. It worked perfectly, and I definitely recommend. From: Yongjin | Date: 26/08/2020 × For differentiation macrophage progenitors. I have been using this human SCF for differentiating macrophage progenitors. It worked perfectly, and I definitely recommend. From: Yongjin | Date: 26/08/2020 Close
