Recombinant Human sFas Receptor 0 ReviewsSubmit a Review Product Details Catalogue Number: 310-20 Description: Fas and Fas Ligand (FasL) belong to the TNF superfamily, and are type I and type II transmembrane proteins, respectively. Binding of FasL to Fas triggers apoptosis in Fas-bearing cells. The mechanism of apoptosis involves recruitment of pro-caspase 8 through an adaptor molecule called FADD, followed by processing of the pro-enzyme into active forms. These active caspases then cleave various cellular substrates, leading to the eventual cell death. sFasR is capable of inhibiting FasL-induced apoptosis by acting as a decoy receptor that serves as a sink for FasL. The full length Fas (receptor) is a 319 amino acid type I transmembrane protein, which contains a 157 amino acid extracellular domain, a 17 amino acid transmembrane domain, and a 145 amino acid cytoplasmic domain. Recombinant Human soluble Fas (sFas Receptor) is a 157 amino acid polypeptide (17.6 kDa) corresponding to the TNFR-homologous cysteine-rich extracellular Fas domain. Source: E.coli Synonyms: soluble Fas receptor (sFasR), TNFRSF6, CD95, Apo I, Fas Antigen AA Sequence: MRLSSKSVNA QVTDINSKGL ELRKTVTTVE TQNLEGLHHD GQFCHKPCPP GERKARDCTV NGDEPDCVPC QEGKEYTDKA HFSSKCRRCR LCDEGHGLEV EINCTRTQNT KCRCKPNFFC NSTVCEHCDP CTKCEHGIIK ECTLTSNTKC KEEGSRS Purity: ≥ 98% by SDS-PAGE gel and HPLC analyses. Biological Activity: The ED50 was determined by its ability to inhibit the cytotoxicity of Jurkat cells is between 10-15 µg/ml in the presence of 2ng/ml of hFasL. Calculated Molecular Weight: 17.6 kDa Accession Number: P25445 Gene ID: 355 Endotoxin: Endotoxin level is < 0.1 ng/ug of protein (< 1 EU/ug) crossreactivity: References PubMed SDS CoA Search Product Line Country Of Origin: USA Not for human use. Research Interest AIDS/HIV Immune System Receptors TNF Superfamily product.subtitle.recentcitations First Author Chodorge, M Title A series of Fas receptor agonist antibodies that demonstrate an inverse correlation between affinity and potency. Citation Cell Death and Differentiation; 19(7) pg1187-95 PubMed Id 22261618
