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Facteurs de Croissance et Cytokines

Recombinant Human TGF-β1 (HEK293 derived)

Product Details

Catalogue Number: 100-21
Recombinant Human TGF-β1 (HEK293 derived)

The three mammalian isoforms of TGF-β, TGF-β1, β2, and β3, signal through the same receptor and elicit similar biological responses. They are multifunctional cytokines that regulate cell proliferation, growth, differentiation and motility, as well as synthesis and deposition of the extracellular matrix. They are involved in various physiological processes, including embryogenesis, tissue remodeling and wound healing. They are secreted predominantly as latent complexes, which are stored at the cell surface and in the extracellular matrix. The release of biologically active TGF-β isoform from a latent complex involves proteolytic processing of the complex and/or induction of conformational changes by proteins such as thrombospondin-1. TGF-β1 is the most abundant isoform secreted by almost every cell type. It was originally identified for its ability to induce phenotypic transformation of fibroblasts, and recently it has been implicated in the formation of skin tumors. Recombinant Human TGF-β1 derived from HEK293 cells is a 25.0 kDa protein with each subunit containing 112 amino acid residues, linked by a single disulfide bond.

Source: HEK293 cells

Synonyms: Transforming Growth Factor-β1, Differentiation inhibiting factor, Cartilage-inducing factor


Purity: ≥ 98% by SDS-PAGE gel and HPLC analyses.

Biological Activity: The ED50 was determined by TGF-beta1's ability to inhibit the mouse IL-4-dependent proliferation of mouse HT-2 cells is ≤ 0.05 ng/ml, corresponding to a specific activity of ≥ 2 x 107 units/mg.

This product has been tested against the WHO standard. International Unit information can be found here.

Recombinant Human TGF-β1 (HEK293 derived) Biological Activity Graph Recombinant Human TGF-β1 (HEK293 derived) WHO Comparison Graph Recombinant Human TGF-β1 (HEK293 derived) Biological Activity Graph Recombinant Human TGF-β1 (HEK293 derived) WHO Comparison Graph Recombinant Human TGF-β1 (HEK293 derived) Biological Activity Graph Recombinant Human TGF-β1 (HEK293 derived) WHO Comparison Graph

Calculated Molecular Weight: 25 kDa

Accession Number: P01137

Gene ID: 7040

Endotoxin: Endotoxin level is < 0.1 ng/ug of protein (< 1 EU/ug)

Country Of Origin: USA

Not for human use.

Research Interest


First Author
Abe, S
Differences in the stemness characteristics and molecular markers of distinct human oral tissue neural crest-derived multilineage cells.
Cell Proliferation; 55(10) pge13286
PubMed Id
First Author
Park, J
Multi-Omics-Based Autophagy-Related Untypical Subtypes in Patients with Cerebral Amyloid Pathology.
Advanced science (Weinheim, Baden-Wurttemberg, Germany); 9(23) pge2201212
PubMed Id
First Author
Otake-Kasamoto, Y
Lysophosphatidylserines derived from microbiota in Crohn's disease elicit pathological Th1 response.
The Journal of Experimental Medicine; 219(7)
PubMed Id


Existing reviews
Good quality price for the best results
We have been using this reagent for years for creating neo cartilage from our chondrocytes and MSCs with very satisfactory results! I'd completely recommend it.
From:  Alejandro | Date:  09/07/2020
For Treg induction
Very good for experiments with induced regulatory T cells, enables efficient induction of human Treg at 10-20ng/ml. Consistent across lots.
From:  Julian | Date:  10/07/2020
Good for EMT induction
We have been using it for years to induce EMT in human primary small airway epithelial cells.
From:  Jun | Date:  21/07/2020
Worked as Described!
From:  Lillian | Date:  21/07/2020
Good and cheap
Works quite well in organoid cultures and it's for a very reasonable price.
From:  Melissa | Date:  29/07/2020
Works well to induce collagen and GAG production in neocartilage
Our group has used this product for years and we are always pleased with the outcome in our systems. Batch to batch variation is very low and works consistently in culturing neocartilage with the intent to repair focal cartilage defects.
From:  Ryan | Date:  21/08/2020
Good quality product
Thank you for providing us good quality cytokines with resonable price and fast delivery. Appreciate your service during Covid times. Quality of the product is excellent.
From:  Deepti | Date:  31/08/2020
Quality Growth Factor for mesenchymal (EMT) signaling
I used for Organoid Culture with mesenchymal signaling.
Needed for squamous type of organoid growth.
From:  Hardik | Date:  31/08/2020
good for pro-inflammatory and pro-fibrotic cocktails
Using this at 3 ng/ml or 5 ng/ml promoted some inflammatory and fibrosis pathways we were investigating. This was very helpful when we needed to evaluate molecular mechanisms of our drug targets.
From:  Vikram | Date:  03/08/2021
Easy in use, easy dissolved
Fine product of Peprotech. We use it diluted - added freshly - in our diffentiation medium and are very content with the product
From:  Helen | Date:  09/08/2021
Good and consistent
We have successfully been using this product to activate fibroblasts in vitro with concentrations from 5 to 20 ng/mL. I highly reccomend it.
From:  Rita | Date:  23/08/2021
Chondrocyte responsive
Worked well for my chondrocyte cell line, induced a good response
From:  Oliver | Date:  25/08/2021