Facteurs de Croissance et Cytokines

Recombinant Human TNF-α

Product Details

Catalogue Number: 300-01A
Description:

TNF-α is a pleiotropic pro-inflammatory cytokine secreted by various cells, including adipocytes, activated monocytes, macrophages, B cells, T cells and fibroblasts. It belongs to the TNF family of ligands, and signals through two receptors, TNFR1 and TNFR2. TNF-α is cytotoxic to a wide variety of tumor cells, and is an essential factor in mediating the immune response against bacterial infections. TNF-α also plays a role in the induction of septic shock, autoimmune diseases, rheumatoid arthritis, inflammation, and diabetes. Human and murine TNF-α demonstrate significant cross-species reactivity. TNF-α exists in two forms; a type II transmembrane protein, and a mature soluble protein. The TNF-α transmembrane protein is proteolitically cleaved to yield a soluble, biologically active, 17 kDa TNF-α, which forms a non-covalently linked homotrimer in solution. Recombinant Human TNF-α is a soluble 157 amino acid protein (17.4 kDa) which corresponds to C-terminal extracellular domain of the full length transmembrane protein.

Source: E.coli

Synonyms: Tumor Necrosis Factor, TNFSF2, Cachectin, Differentiation-inducing factor (DIF), Necrosin, Cytotoxin

AA Sequence: VRSSSRTPSD KPVAHVVANP QAEGQLQWLN RRANALLANG VELRDNQLVV PSEGLYLIYS QVLFKGQGCP STHVLLTHTI SRIAVSYQTK VNLLSAIKSP CQRETPEGAE AKPWYEPIYL GGVFQLEKGD RLSAEINRPD YLDFAESGQV YFGIIAL

Purity: ≥ 98% by SDS-PAGE gel and HPLC analyses.

Biological Activity: The ED50 as determined by the cytolysis of murine L929 cells in the presence of Actinomycin D is ≤ 0.05 ng/ml, corresponding to a specific activity of ≥ 2 x 107 units/mg.

Calculated Molecular Weight: 17.4 kDa

Accession Number: P01375

Gene ID: 7124

crossreactivity:
Country Of Origin: USA

Not for human use.

Research Interest

product.subtitle.productreviews

Existing reviews
Great recombinant protein from peprotech
We use this recombinant TNFa in one of our assays and it has performed extremely well/consistent over the years. I would definitely recommend this product to people who are interested.
From:  Di | Date:  08/07/2020
Reliable TNF both for human and mouse
Using this item for over 12 years.
Always gives consistent results.
Note- very sensitive to freezing and thawing.
We keep it in 100ug/ml, 10ul aliquotes for use and minimize repeated freeze/thaw of the vial.
Works well at 10 ng/ml - 100 ng/ml to study inflammatory gene expression.
From:  Param | Date:  09/07/2020
in vitro probiotic properties assay
We routinely use recombinant human TNF-α in co-incubation with HT29 cells to characterization in vitro of anti-inflammatory probiotic properties of potential candidate strains used IL-8 as readout. This product as Prepothec indicates is sensitive to thawed cycles, I recommend prepare aliquots (100ng/ul).
From:  Edgar | Date:  26/07/2020
Easy to reconstitute and store for long-term usage
This is useful in some of our inflammation and fibrosis studies. It has a decent shelf like in -20C of few months and gets used well before then. Recommendation is to make aliquots of 50-100 ul of 10 ug/ml stocks.
From:  Vikram | Date:  03/08/2021