Recombinant Human TPO 1 ReviewsSubmit a Review Product Details Catalogue Number: 300-18 Description: TPO is a lineage-specific growth factor produced in the liver, kidney and skeletal muscle. It stimulates the proliferation and maturation of megakaryocytes, and promotes increased circulating levels of platelets in vivo. TPO signals through the c-mpl receptor, and acts as an important regulator of circulating platelets. Human and murine TPO exhibit cross-species reactivity. The human TPO gene encodes for a 353 amino acid glycoprotein, which contains a 21 amino acid signal sequence, a 15 amino acid erythropoietin-like domain, and a highly glycosylated 179 amino acid C-terminal domain. Recombinant Human TPO is a fully biologically active 174 amino acid polypeptide (18.6 kDa), which contains the erythropoietin-like domain of the full length TPO protein. Source: E.coli Synonyms: Thrombopoietin, Megakaryocyte colony-stimulating factor, c-MPL Ligand, MGDF AA Sequence: SPAPPACDLR VLSKLLRDSH VLHSRLSQCP EVHPLPTPVL LPAVDFSLGE WKTQMEETKA QDILGAVTLL LEGVMAARGQ LGPTCLSSLL GQLSGQVRLL LGALQSLLGT QLPPQGRTTA HKDPNAIFLS FQHLLRGKVR FLMLVGGSTL CVRRAPPTTA VPSRTSLVLT LNEL Purity: ≥ 98% by SDS-PAGE gel and HPLC analyses. Biological Activity: The ED50 as determined by the dose-dependent stimulation of the proliferation of human MO7e cells was found to be ≤ 1.0 ng/ml, corresponding to a specific activity of ≥ 1 x 106 units/mg. Calculated Molecular Weight: 18.6 kDa Accession Number: P40225 Gene ID: 7066 crossreactivity: Human, Human + Hamster, Human + Monkey, Human + Mouse, Monkey, Mouse, Mouse + Virus, Rat References PubMed SDS CoA Search Product Line Country Of Origin: USA Not for human use. Research Interest Cell Culture Immune System Stem Cells & Differentiation Wound Healing product.subtitle.recentcitations First Author Goff, J P Title Influence of cytokines on the growth kinetics and immunophenotype of daughter cells resulting from the first division of single CD34(+)Thy-1(+)lin- cells. Citation Blood; 92(11) pg4098-107 PubMed Id 9834215 First Author Simeoni, F Title Enhancer recruitment of transcription repressors RUNX1 and TLE3 by mis-expressed FOXC1 blocks differentiation in acute myeloid leukemia. Citation Cell Reports; 36(12) pg109725 PubMed Id 34551306 First Author Dignum, T Title Multipotent progenitors and hematopoietic stem cells arise independently from hemogenic endothelium in the mouse embryo. Citation Cell Reports; 36(11) pg109675 PubMed Id 34525376 product.subtitle.productreviews Existing reviews TPO dans la culture Cellules souches pluripotentes J'ai utilise de la TPOhu pour la différenciation des cellules souches pluripotentes humaines vers l'hemangioblaste et les cellules hématopoïètiques.Je suis très satisfaite de ce produit (et depuis plusieures années) From: Maite | Date: 29/07/2020 × TPO dans la culture Cellules souches pluripotentes J'ai utilise de la TPOhu pour la différenciation des cellules souches pluripotentes humaines vers l'hemangioblaste et les cellules hématopoïètiques.Je suis très satisfaite de ce produit (et depuis plusieures années) From: Maite | Date: 29/07/2020 Close
