Facteurs de Croissance et Cytokines

Recombinant Human Wnt-7a

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Product Details

Catalogue Number: 120-31
Description:
Recombinant Human Wnt-7a

Wnt-7a belongs to the Wnt family of signaling proteins that play a key role in maintaining the integrity of embryonic and adult tissues. It is expressed in placenta, kidney, testis, uterus, fetal lung, and fetal and adult brain. Most Wnt proteins can signal though a mechanism called the canonical Wnt pathway, in which Wnt proteins bind to and activate seven-pass transmembrane receptors of the Frizzled family, ultimately leading to the disruption of β-catenin degradation. Intracellular accumulation of β-catenin increases translocation of the protein into the nucleus, where it binds to TCF/LEF transcription factors to induce the expression of numerous genes. Increased Wnt/β-catenin signaling is associated with tumorigenesis in a diverse set of human cancers. However, Wnt-7a/Frizzled-9 signaling has been shown to act as a tumor suppressor in non-small cell lung cancers. Recombinant Human Wnt-7a is a 35.5 kDa glycoprotein containing 318 amino acids. Due to glycosylation, Wnt-7a migrates between 40-55 kDa by SDS-PAGE gel under unreduced conditions.

Source: HEK293 cells

Synonyms: None.

AA Sequence: LGASIICNKI PGLAPRQRAI CQSRPDAIIV IGEGSQMGLD ECQFQFRNGR WNCSALGERT VFGKELKVGS REAAFTYAII AAGVAHAITA ACTQGNLSDC GCDKEKQGQY HRDEGWKWGG CSADIRYGIG FAKVFVDARE IKQNARTLMN LHNNEAGRKI LEENMKLECK CHGVSGSCTT KTCWTTLPQF RELGYVLKDK YNEAVHVEPV RASRNKRPTF LKIKKPLSYR KPMDTDLVYI EKSPNYCEED PVTGSVGTQG RACNKTAPQA SGCDLMCCGR GYNTHQYARV WQCNCKFHWC CYVKCNTCSE RTEMYTCK

Purity: ≥ 80% by SDS-PAGE gel and HPLC analyses.

Biological Activity: Determined by its ability to decrease alkaline phosphatase activity in CCL-226 cells when treated with 25 ng/ml of Murine Wnt-3a.

Calculated Molecular Weight: 35.5 kDa

Accession Number: O00755

Gene ID: 7476

Note:

100μg is the largest vial size for this product.
Larger sizes supplied as multiple 100μg vials.

crossreactivity:
Country Of Origin: USA

Not for human use.

Research Interest

product.subtitle.recentcitations

First Author
Huang, X
Title
Wnt7a activates canonical Wnt signaling, promotes bladder cancer cell invasion, and is suppressed by miR-370-3p.
Citation
The Journal of Biological Chemistry; 293(18) pg6693-6706
PubMed Id
First Author
Li, A
Title
Shaping organs by a wingless-int/Notch/nonmuscle myosin module which orients feather bud elongation.
Citation
Proceedings of the National Academy of Sciences of the United States of America; 110(16) pgE1452-61
PubMed Id