Recombinant Murine M-CSF

Product Details

Catalogue Number: 315-02

Description:

M-CSF is a potent hematopoietic factor produced by a variety of cells, including lymphocytes, monocytes, fibroblasts, endothelial cells, myoblasts and osteoblasts. It is a key regulator of cellular proliferation, differentiation, and survival for blood monocytes, tissue macrophages, and their respective progenitor cells. M-CSF has been shown to play important roles in modulating dermal thickness and fertility. M-CSF is clinically used in the treatment of infection, malignancies and atherosclerosis. It facilitates hematopoietic recovery after bone marrow transplantation. Human M-CSF is reactive in murine systems, but the murine molecule exhibits no activity on human cells. Recombinant Murine M-CSF is a 36.4 kDa homodimeric protein consisting of two 156 amino acid polypeptide subunits.

Source: E.coli

Synonyms: Macrophage Colony Stimulating Factor, CSF-1, MGI-IM

AA Sequence (monomer): MKEVSEHCSH MIGNGHLKVL QQLIDSQMET SCQIAFEFVD QEQLDDPVCY LKKAFFLVQD IIDETMRFKD NTPNANATER LQELSNNLNS CFTKDYEEQN KACVRTFHET PLQLLEKIKN FFNETKNLLE KDWNIFTKNC NNSFAKCSSR DVVTKP

Purity: ≥ 98% by SDS-PAGE gel and HPLC analyses.

Biological Activity: The ED₅₀ as determined by the dose-dependent stimulation of the proliferation of M-NFS-60 cells is ≤ 1.0 ng/ml, corresponding to a specific activity of ≥ 1 x 10⁶ units/mg.

Calculated Molecular Weight: 36.4 kDa

Accession Number: P07141

Gene ID: 12977

Endotoxin: Endotoxin level is < 0.1 ng/ug of protein (< 1 EU/ug)

crossreactivity:

Cow + Mouse, Human, Mouse, Rat

Country Of Origin: USA

Not for human use.

Research Interest

Cancer

Immune System

Stem Cells & Differentiation

product.subtitle.recentcitations

First Author

Alexandrou, A T

Title

PERIOD 2 regulates low-dose radioprotection via PER2/pGSK3β/β-catenin/Per2 loop.

Citation

Iscience; 25(12) pg105546

PubMed Id

36465103

First Author

Sharma, A

Title

Glycolipid Metabolite β-Glucosylceramide Is a Neutrophil Extracellular Trap-Inducing Ligand of Mincle Released during Bacterial Infection and Inflammation.

Citation

Journal of immunology (Baltimore, Md. : 1950); 209(2) pg391-400

PubMed Id

35768151

First Author

Huang, M

Title

miR-134-5p inhibits osteoclastogenesis through a novel miR-134-5p/Itgb1/MAPK pathway.

Citation

The Journal of Biological Chemistry; 298(7) pg102116

PubMed Id

35691339

product.subtitle.productreviews

Existing reviews

Differencitaion of bone marrow progenitors in bone marrow derived macrophages

I used murine M-CSF to differentiate bone marrow progenitors in bone marrow derived macrophages.

From: Laura | Date: 28/07/2020

MCSF

Good product. Works well to generate macrophages from BM.

From: Kevin | Date: 26/08/2020

Excellent product

I have used this product for differentiating bone marrow-derived monocytes to macrophages. It works very well based on visual observations as well as flow cytometry data on macrophage surface protein expression. I use it very often for my project.

From: Kiran | Date: 26/08/2020

M-CSF for maintaining mouse microglial lines

I have been using murine M-CSF for maintaining mouse microglial lines, EOC20. And it worked perfectly. Definitely recommend.

From: Yongjin | Date: 26/08/2020

Facteurs de Croissance et Cytokines