Animal-Free Recombinant Murine M-CSF

Détails du Produit

Catalog Number: AF-315-02

Description:

M-CSF is a potent hematopoietic factor produced by a variety of cells, including lymphocytes, monocytes, fibroblasts, endothelial cells, myoblasts and osteoblasts. It is a key regulator of cellular proliferation, differentiation, and survival for blood monocytes, tissue macrophages, and their respective progenitor cells. M-CSF has been shown to play important roles in modulating dermal thickness and fertility. M-CSF is clinically used in the treatment of infection, malignancies and atherosclerosis. It facilitates hematopoietic recovery after bone marrow transplantation. Human M-CSF is reactive in murine systems, but the murine molecule exhibits no activity on human cells. Recombinant Murine M-CSF is a 36.4 kDa homodimeric protein consisting of two 156 amino acid polypeptide subunits.

Manufactured using all Animal-Free reagents.

Source: E.coli

Synonyms: Macrophage Colony Stimulating Factor, CSF-1, MGI-IM

AA Sequence (monomer): MKEVSEHCSH MIGNGHLKVL QQLIDSQMET SCQIAFEFVD QEQLDDPVCY LKKAFFLVQD IIDETMRFKD NTPNANATER LQELSNNLNS CFTKDYEEQN KACVRTFHET PLQLLEKIKN FFNETKNLLE KDWNIFTKNC NNSFAKCSSR DVVTKP

Purity: ≥ 98% by SDS-PAGE gel and HPLC analyses.

Biological Activity: Determined by its ability to stimulate the proliferation of murine M-NFS-60 cells. The expected ED₅₀ is ≤ 1.0 ng/ml, corresponding to a specific activity of ≥ 1 x 10⁶ units/mg.

Calculated Molecular Weight: 36.4 kDa

Accession Number: P07141

Gene ID: 12977

Endotoxin: Endotoxin level is < 0.01 ng/ug of protein (< 0.1 EU/ug)

crossreactivity:

Mouse, Rat