Facteurs de Croissance et Cytokines

Recombinant Murine TNF-α

Product Details

Catalogue Number: 315-01A
Description:

TNF-α is a pleiotropic pro-inflammatory cytokine secreted by various cells including adipocytes, activated monocytes, macrophages, B cells, T cells and fibroblasts. It belongs to the TNF family of ligands and signals through two receptors, TNFR1 and TNFR2. TNF-α is cytotoxic to a wide variety of tumor cells and is an essential factor in mediating the immune response against bacterial infections. TNF-α also plays a role in the induction of septic shock, auto immune diseases, rheumatoid arthritis, inflammation, and diabetes. Recombinant Murine TNF-α is a soluble 156 amino acid protein (17.3 kDa) which corresponds to C-terminal extracellular domain of the full length transmembrane protein.

Source: E.coli

Synonyms: Tumor Necrosis Factor, TNFSF2, Cachectin, Differentiation-inducing factor (DIF), Necrosin, Cytotoxin

AA Sequence: MLRSSSQNSS DKPVAHVVAN HQVEEQLEWL SQRANALLAN GMDLKDNQLV VPADGLYLVY SQVLFKGQGC PDYVLLTHTV SRFAISYQEK VNLLSAVKSP CPKDTPEGAE LKPWYEPIYL GGVFQLEKGD QLSAEVNLPK YLDFAESGQV YFGVIAL

Purity: ≥ 98% by SDS-PAGE gel and HPLC analyses.

Biological Activity: The ED50 as determined by the cytolysis of murine L929 cells in the presence of actinomycin D is ≤ 0.1 ng/ml, corresponding to a specific activity of  ≥ 1 x 107 units/mg.

Calculated Molecular Weight: 17.3 kDa

Accession Number: P06804

Gene ID: 21926

crossreactivity:
Country Of Origin: USA

Not for human use.

Research Interest

product.subtitle.recentcitations

First Author
Mócsai, A
Title
Adhesion-dependent degranulation of neutrophils requires the Src family kinases Fgr and Hck.
Citation
Journal of immunology (Baltimore, Md. : 1950); 162(2) pg1120-6
PubMed Id
First Author
Munder, M
Title
Murine macrophages secrete interferon gamma upon combined stimulation with interleukin (IL)-12 and IL-18: A novel pathway of autocrine macrophage activation.
Citation
The Journal of Experimental Medicine; 187(12) pg2103-8
PubMed Id
First Author
Sakai, S
Title
Inhibitory effect of ferulic acid on macrophage inflammatory protein-2 production in a murine macrophage cell line, RAW264.7.
Citation
Cytokine; 9(4) pg242-8
PubMed Id