Antigen Affinity Purified Polyclonal Antibodies

Anti-Human CTGF

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Product Details

Catalogue Number: 500-P252

Source: Polyclonal Rabbit

Preparation: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hCTGF. Anti-Human CTGF specific antibody was purified by affinity chromatography employing immobilized hCTGF matrix.

Immunogen: E.coli derived Recombinant Human CTGF (PeproTech catalog# 120-19)

Sandwich ELISA: To detect hCTGF by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech’s Biotinylated Anti-Human CTGF (500-P252Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hCTGF.

Anti-Human CTGF Sandwich ELISA

Western Blot: To detect hCTGF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hCTGF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Anti-Human CTGF Western Blot Reduced Anti-Human CTGF Western Blot Unreduced

Note:

Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information.

crossreactivity:
Country Of Origin: USA

Not for human use.

Research Interest

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Yang, S
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P38 pathway as a key downstream signal of connective tissue growth factor to regulate metastatic potential in non-small-cell lung cancer
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