Produced from sera of rabbits immunized with highly pure Recombinant Human FGF-23. Anti-Human FGF-23-specific antibody was purified by affinity chromatography employing an immobilized Human FGF-23 matrix.
E.coli-derived, 25.5kDa Recombinant Human FGF-23 (PeproTech Catalog# 100-52)
To detect Human FGF-23 by sandwich ELISA (using 100ul/well), a concentration of 0.5-2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech’s Biotinylated Anti-Human FGF-23 (500-P319BT) as a detection antibody, allows the detection of at least 2000-4000 pg/ml of Recombinant Human FGF-23.
To detect Human FGF-23 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Human FGF-23 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information.
Not for human use.