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Antigen Affinity Purified Polyclonal Antibodies

Anti-Human Galectin-1

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Product Details

Catalog Number: 500-P210
Description:
Anti-Human Galectin-1
 

Source: Polyclonal Rabbit

Preparation: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hGalectin-1. Anti-Human Galectin-1 specific antibody was purified by affinity chromatography employing immobilized hGalectin-1 matrix.

Immunogen: E.coli derived Recombinant Human Galectin-1 (PeproTech catalog# 450-39)

Sandwich ELISA: To detect hGalectin-1 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech’s Biotinylated Anti-Human Galectin-1 (500-P210Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hGalectin-1.

Anti-Human Galectin-1 Sandwich ELISA

Western Blot: To detect hGalectin-1 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hGalectin-1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Anti-Human Galectin-1 Western Blot Reduced Anti-Human Galectin-1 Western Blot Unreduced

Note:

Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information.

Cross Reactivity Cited in References:
Country Of Origin: USA

Not for human use.

Research Interest

Recent Citations

First Author
Sanjurjo, L
Title
Chemokines modulate glycan binding and the immunoregulatory activity of galectins.
Citation
Communications Biology; 4(1) pg1415
PubMed ID
First Author
Posada, I M
Title
Opposite feedback from mTORC1 to H-ras and K-ras4B downstream of SREBP1.
Citation
Scientific Reports; 7(1) pg8944
PubMed ID
First Author
Blaževitš, O
Title
Galectin-1 dimers can scaffold Raf-effectors to increase H-ras nanoclustering.
Citation
Scientific Reports; 6 pg24165
PubMed ID