Antigen Affinity Purified Polyclonal Antibodies

Anti-Human IGF-BP5

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Product Details

Catalogue Number: 500-P232
Anti-Human IGF-BP5

Source: Polyclonal Rabbit

Preparation: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hIGF-BP5. Anti-Human IGF-BP5 specific antibody was purified by affinity chromatography employing immobilized hIGF-BP5 matrix.

Immunogen: E.coli derived Recombinant Human IGF-BP5 (PeproTech catalog# 100-05)

Sandwich ELISA: To detect hIGF-BP5 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech’s Biotinylated Anti-Human IGF-BP5 (500-P232Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIGF-BP5.

Anti-Human IGF-BP5 Sandwich ELISA

Western Blot: To detect hIGF-BP5 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIGF-BP5 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Anti-Human IGF-BP5 Western Blot Reduced Anti-Human IGF-BP5 Western Blot Unreduced


Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information.

Country Of Origin: USA

Not for human use.

Research Interest


First Author
Fatrai, S
KRAS(G12V) enhances proliferation and initiates myelomonocytic differentiation in human stem/progenitor cells via intrinsic and extrinsic pathways.
The Journal of Biological Chemistry; 286(8) pg6061-70
PubMed Id