Antigen Affinity Purified Polyclonal Antibodies

Anti-Human IL-6 (Polyclonal Goat)

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Product Details

Catalogue Number: 500-P26G

Source: Polyclonal Goat

Preparation: Produced from sera of goats pre-immunized with highly pure recombinant Human IL-6. Anti-Human IL-6 specific antibody was purified by affinity chromatography employing immobilized Human IL-6 matrix.

Immunogen: E.coli derived Recombinant Human IL-6 (PeproTech catalog# 200-06)

Sandwich ELISA: To detect Human IL-6 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech’s Biotinylated Anti-Human IL-6 (500-P26GBt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human IL-6.

Anti-Human IL-6 Sandwich ELISA

Western Blot: To detect Human IL-6 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. When used in conjunction with compatible secondary reagents the detection limit for recombinant Human IL-6 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Anti-Human IL-6 Western Blot Reduced Anti-Human IL-6 Western Blot Unreduced

Note:

Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information.

crossreactivity:
Country Of Origin: USA

Not for human use.

Research Interest

product.subtitle.recentcitations

First Author
Edsbäcker, E
Title
STAT3 is activated in multicellular spheroids of colon carcinoma cells and mediates expression of IRF9 and interferon stimulated genes.
Citation
Stem Cell Reports; 9(1) pg536
PubMed Id
First Author
Khatib, A
Title
The glutathione peroxidase 8 (GPX8)/IL-6/STAT3 axis is essential in maintaining an aggressive breast cancer phenotype.
Citation
Proceedings of the National Academy of Sciences of the United States of America; 117(35) pg21420-21431
PubMed Id