Antigen Affinity Purified Polyclonal Antibodies

Anti-Human LEC (CCL16)

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Product Details

Catalogue Number: 500-P125G

Source: Polyclonal Goat

Preparation: Produced from sera of goats pre-immunized with highly pure (>98%) recombinant hLEC. Anti-Human LEC specific antibody was purified by affinity chromatography employing immobilized hLEC matrix.

Immunogen: E.coli derived Recombinant Human LEC (CCL16) (PeproTech catalog# 300-44)

Sandwich ELISA: To detect hLEC by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech’s Biotinylated Anti-Human LEC (500-P125GBt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hLEC.

Anti-Human LEC (CCL16) Sandwich ELISA

Western Blot: To detect hLEC by Western Blot analysis this antibody can be used at a concentration of 0.1- 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hLEC is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Anti-Human LEC (CCL16) Western Blot Reduced Anti-Human LEC (CCL16) Western Blot Unreduced

Note:

Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information.

crossreactivity:
Country Of Origin: USA

Not for human use.

Research Interest

product.subtitle.recentcitations

First Author
Giovarelli, M
Title
Tumor rejection and immune memory elicited by locally released LEC chemokine are associated with an impressive recruitment of APCs, lymphocytes, and granulocytes.
Citation
Journal of immunology (Baltimore, Md. : 1950); 164(6) pg3200-6
PubMed Id
First Author
Howard, O M
Title
LEC induces chemotaxis and adhesion by interacting with CCR1 and CCR8.
Citation
Blood; 96(3) pg840-5
PubMed Id