Antigen Affinity Purified Polyclonal Antibodies

Anti-Human Leptin

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Product Details

Catalogue Number: 500-P86

Source: Polyclonal Rabbit

Preparation: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hLeptin. Anti-Human Leptin specific antibody was purified by affinity chromatography employing immobilized hLeptin matrix.

Immunogen: E.coli derived Recombinant Human Leptin (PeproTech catalog# 300-27)

Sandwich ELISA: To detect hLeptin by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech’s Biotinylated Anti-Human Leptin (500-P86Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hLeptin.

Anti-Human Leptin Sandwich ELISA

Western Blot: To detect hLeptin by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hLeptin is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Anti-Human Leptin Western Blot Reduced Anti-Human Leptin Western Blot Unreduced


Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information.

Country Of Origin: USA

Not for human use.

Research Interest


First Author
Huan, J
Adipocyte-selective reduction of the leptin receptors induced by antisense RNA leads to increased adiposity, dyslipidemia, and insulin resistance.
The Journal of Biological Chemistry; 278(46) pg45638-50
PubMed Id
First Author
Dorneles, G P
Immunoregulation induced by autologous serum collected after acute exercise in obese men: a randomized cross-over trial
Scientific Reports; 10(1) pg21735
PubMed Id
First Author
Glasow, A
Expression of leptin (Ob) and leptin receptor (Ob-R) in human fibroblasts: regulation of leptin secretion by insulin.
The Journal of Clinical Endocrinology and Metabolism; 86(9) pg4472-9
PubMed Id