Monoclonal Antibodies

Anti-Human MCP-1 (CCL2) (Monoclonal Mouse)

Product Details

Catalogue Number: 500-M71

Source: Monoclonal Mouse

Preparation: Produced in BALB/c mice immunized with highly pure Recombinant Human MCP-1 (CCL2). Anti-Human MCP-1 (CCL2)-specific antibody was purified from cell culture supernatant by Protein A affinity chromatography.

Immunogen: E.coli derived Recombinant Human MCP-1 (CCL2) (PeproTech catalog# 300-04)

Sandwich ELISA: In a sandwich ELISA (assuming 100μl/well), a concentration of 1.0-2.0 μg/ml of this antibody will detect at least 100pg/ml of Recombinant Human MCP-1 when used with PeproTech’s Biotinylated Antigen Affinity Purified anti-Human MCP-1 (CCL2) (500-P34BT) as the detection antibody at a concentration of approximately 0.5-1.0 μg/ml.

Anti-Human MCP-1 (CCL2) Sandwich ELISA Anti-Human MCP-1 (CCL2) Sandwich ELISA

Western Blot: To detect Human MCP-1 by Western Blot analysis this antibody can be used at a concentration of 0.20-0.40 μg/ml. When used in conjunction with compatible secondary reagents the detection limit for Recombinant Human MCP-1 is 1.0-2.0 ng/lane under reducing conditions and 0.25-0.50 ng/lane under non-reducing conditions.

Anti-Human MCP-1 (CCL2) Western Blot Reduced Anti-Human MCP-1 (CCL2) Western Blot Unreduced Anti-Human MCP-1 (CCL2) Western Blot Reduced Anti-Human MCP-1 (CCL2) Western Blot Unreduced

Note:

Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information.

crossreactivity:
Country Of Origin: USA

Not for human use.

Research Interest

product.subtitle.recentcitations

First Author
Kakizaki, Y
Title
Production of monocyte chemoattractant protein-1 by bovine glomerular endothelial cells.
Citation
Kidney International; 48(6) pg1866-74
PubMed Id
First Author
Fantuzzi, L
Title
Endogenous CCL2 (monocyte chemotactic protein-1) modulates human immunodeficiency virus type-1 replication and affects cytoskeleton organization in human monocyte-derived macrophages.
Citation
Blood; 102(7) pg2334-7
PubMed Id