Antigen Affinity Purified Polyclonal Antibodies

Anti-Human MDC (CCL22)

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Product Details

Catalogue Number: 500-P107

Source: Polyclonal Rabbit

Preparation: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hMDC (human Macrophage-Derived Chemokine). Anti-Human MDC specific antibody was purified by affinity chromatography employing immobilized hMDC matrix.

Immunogen: E.coli derived Recombinant Human MDC (CCL22) (69 a.a.) (PeproTech catalog# 300-36A)

Sandwich ELISA: To detect hMDC by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech’s Biotinylated Anti-Human MDC (500-P107Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hMDC.

Anti-Human MDC (CCL22) Sandwich ELISA

Western Blot: To detect hMDC by Western Blot analysis this antibody can be used at a concentration of 0.1- 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMDC is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Anti-Human MDC (CCL22) Western Blot Reduced Anti-Human MDC (CCL22) Western Blot Unreduced

Note:

Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information.

crossreactivity:
Country Of Origin: USA

Not for human use.

Research Interest

product.subtitle.recentcitations

First Author
Cocchi, F
Title
Soluble factors from T cells inhibiting X4 strains of HIV are a mixture of β chemokines and RNases.
Citation
Proceedings of the National Academy of Sciences of the United States of America; 109(14) pg5411-6
PubMed Id
First Author
Cattoretti, G
Title
Nuclear and cytoplasmic AID in extrafollicular and germinal center B cells.
Citation
Blood; 107(10) pg3967-75
PubMed Id
First Author
Rawal, S
Title
Cross talk between follicular Th cells and tumor cells in human follicular lymphoma promotes immune evasion in the tumor microenvironment.
Citation
Journal of immunology (Baltimore, Md. : 1950); 190(12) pg6681-93
PubMed Id