Antigen Affinity Purified Polyclonal Antibodies

Anti-Human PDGF-BB

0 ReviewsSubmit a Review

Product Details

Catalogue Number: 500-P47

Source: Polyclonal Rabbit

Preparation: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hPDGF-BB. Anti-Human PDGF-BB specific antibody was purified by affinity chromatography employing immobilized hPDGF-BB matrix.

Immunogen: E.coli derived Recombinant Human PDGF-BB (PeproTech catalog# 100-14B)

Sandwich ELISA: To detect hPDGF-BB by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech’s Biotinylated Anti-Human PDGF-BB (500-P47Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hPDGF-BB.

Anti-Human PDGF-BB Sandwich ELISA

Western Blot: To detect hPDGF-BB by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hPDGF-BB is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Anti-Human PDGF-BB Western Blot Reduced Anti-Human PDGF-BB Western Blot Unreduced

Note:

Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information.

crossreactivity:
Country Of Origin: USA

Not for human use.

Research Interest

product.subtitle.recentcitations

First Author
Tan, S H
Title
Regulation of cell proliferation and migration by TAK1 via transcriptional control of von Hippel-Lindau tumor suppressor.
Citation
The Journal of Biological Chemistry; 284(27) pg18047-58
PubMed Id