Antigen Affinity Purified Polyclonal Antibodies

Anti-Human PF-4 (CXCL4)

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Product Details

Catalogue Number: 500-P05

Source: Polyclonal Rabbit

Preparation: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hPF-4. Anti-Human PF-4 specific antibody was purified by affinity chromatography employing immobilized hPF-4 matrix.

Immunogen: E.coli derived Recombinant Human PF-4 (CXCL4) (PeproTech catalog# 300-16)

Sandwich ELISA: To detect Human PF-4 by sandwich ELISA (using 100μl/well) a concentration of 0.5-2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech’s Biotinylated Anti-Human PF-4 (CXCL4) (500-P05BT) as a detection antibody, allows the detection of at least 2000-4000 pg/ml of Recombinant Human PF-4.

Anti-Human PF-4 (CXCL4) Sandwich ELISA

Western Blot: To detect Human PF-4 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/ml. When used in conjunction with compatible secondary reagents the detection limit for Recombinant Human PF-4 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Anti-Human PF-4 (CXCL4) Western Blot Reduced Anti-Human PF-4 (CXCL4) Western Blot Unreduced

Note:

Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information.

crossreactivity:
Country Of Origin: USA

Not for human use.

Research Interest

product.subtitle.recentcitations

First Author
Johnston, I
Title
Recognition of PF4-VWF complexes by heparin-induced thrombocytopenia antibodies contributes to thrombus propagation.
Citation
Blood; 135(15) pg1270-1280
PubMed Id
First Author
Shi, G
Title
Platelet factor 4 mediates vascular smooth muscle cell injury responses.
Citation
Blood; 121(21) pg4417-27
PubMed Id
First Author
Nassar, T
Title
Platelet factor 4 enhances the binding of oxidized low-density lipoprotein to vascular wall cells.
Citation
The Journal of Biological Chemistry; 278(8) pg6187-93
PubMed Id