Antigen Affinity Purified Polyclonal Antibodies

Anti-Human RANTES (CCL5)

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Product Details

Catalogue Number: 500-P36

Source: Polyclonal Rabbit

Preparation: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hRANTES. Anti-Human RANTES specific antibody was purified by affinity chromatography employing immobilized hRANTES matrix.

Immunogen: E.coli derived Recombinant Human RANTES (CCL5) (PeproTech catalog# 300-06)

Sandwich ELISA: To detect hRANTES by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech’s Biotinylated Anti-Human RANTES (500-P36Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hRANTES.

Anti-Human RANTES (CCL5) Sandwich ELISA

Western Blot: To detect hRANTES by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hRANTES is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Anti-Human RANTES (CCL5) Western Blot Reduced Anti-Human RANTES (CCL5) Western Blot Unreduced

Note:

Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information.

crossreactivity:
Country Of Origin: USA

Not for human use.

Research Interest

product.subtitle.recentcitations

First Author
Secchi, M
Title
Rational CCL5 mutagenesis integration in a lactobacilli platform generates extremely potent HIV-1 blockers.
Citation
Scientific Reports; 8(1) pg1890
PubMed Id
First Author
Øynebråten, I
Title
Oligomerized, filamentous surface presentation of RANTES/CCL5 on vascular endothelial cells.
Citation
Scientific Reports; 5 pg9261
PubMed Id
First Author
Rius, C
Title
Trans- but not cis-resveratrol impairs angiotensin-II-mediated vascular inflammation through inhibition of NF-κB activation and peroxisome proliferator-activated receptor-gamma upregulation.
Citation
Journal of immunology (Baltimore, Md. : 1950); 185(6) pg3718-27
PubMed Id