Anti-Human sCD40 Ligand 0 ReviewsSubmit a Review Product Details Catalog Number: 500-P142G Description: Source: Polyclonal Goat Preparation: Produced from sera of goats pre-immunized with highly pure (>98%) recombinant hsCD40L. Anti-Human sCD40L specific antibody was purified by affinity chromatography employing immobilized hsCD40L matrix. Immunogen: E.coli derived Recombinant Human sCD40 Ligand (PeproTech catalog# 310-02) Sandwich ELISA: To detect hsCD40 Ligand by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech’s Biotinylated Anti-Human sCD40 Ligand (500-P142GBt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hsCD40 Ligand. Western Blot: To detect hsCD40 Ligand by Western Blot analysis this antibody can be used at a concentration of 0.1- 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hsCD40 Ligand is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. Note: Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information. Cross Reactivity Cited in References: Human References SDS CoA Search Product Line Country Of Origin: USA Not for human use. Research Interest Organoids Angiogenesis/Cardiovascular Apoptosis Immune System Inflammation Proliferation TNF Superfamily Allergy Transplantation Recent Citations First Author Aranburu, A Title TLR ligation triggers somatic hypermutation in transitional B cells inducing the generation of IgM memory B cells. Citation Journal of immunology (Baltimore, Md. : 1950); 185(12) pg7293-301 PubMed ID 21078901
