Antigen Affinity Purified Polyclonal Antibodies

Anti-Human sFas Ligand

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Product Details

Catalogue Number: 500-P184G

Source: Polyclonal Goat

Preparation: Produced from sera of goats pre-immunized with highly pure (>98%) recombinant hsFasL/Apo1L. Anti-Human sFasL/Apo1L specific antibody was purified by affinity chromatography employing immobilized hsFasL/Apo1L matrix.

Immunogen: CHO cells derived Recombinant Human sFas Ligand (PeproTech catalog# 310-03)*
*Catalog# 310-03 has been replaced with 310-03H.

Sandwich ELISA: To detect hsFasL/Apo1L by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech’s Biotinylated Anti-Human sFasL/Apo1L (500-P184GBt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hsFasL/Apo1L.

Anti-Human sFas Ligand Sandwich ELISA Anti-Human sFas Ligand Sandwich ELISA

Western Blot: To detect hsFasL/Apo1L by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hsFasL/Apo1L is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Anti-Human sFas Ligand Western Blot Reduced Anti-Human sFas Ligand Western Blot Unreduced Anti-Human sFas Ligand Western Blot Reduced Anti-Human sFas Ligand Western Blot Unreduced

Note:

Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information.

crossreactivity:
Country Of Origin: USA

Not for human use.

Research Interest

product.subtitle.recentcitations

First Author
Subramanyam, M
Title
Inhibition of protein kinase Akt1 by apoptosis signal-regulating kinase-1 (ASK1) is involved in apoptotic inhibition of regulatory volume increase.
Citation
The Journal of Biological Chemistry; 285(9) pg6109-17
PubMed Id
First Author
Komarov, A P
Title
Functional genetics-directed identification of novel pharmacological inhibitors of FAS- and TNF-dependent apoptosis that protect mice from acute liver failure.
Citation
Cell death & disease; 7 pge2145
PubMed Id
First Author
Herrero, R
Title
The biological activity of FasL in human and mouse lungs is determined by the structure of its stalk region.
Citation
The Journal of Clinical Investigation; 121(3) pg1174-90
PubMed Id