Antigen Affinity Purified Polyclonal Antibodies

Anti-Human TGF-α

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Product Details

Catalogue Number: 500-P16

Source: Polyclonal Rabbit

Preparation: Produced from sera of rabbits pre-immunized with highly pure recombinant Human TGF-α. Anti-Human TGF-α specific antibody was purified by affinity chromatography employing immobilized Human TGF-α matrix.

Immunogen: E.coli derived Recombinant Human TGF-α (PeproTech catalog# 100-16A)

Sandwich ELISA: To detect Human TGF-α by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech’s Biotinylated Anti-Human TGF-α (500-P16Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human TGF-α.

Anti-Human TGF-α Sandwich ELISA

Western Blot: To detect Human TGF-α by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human TGF-α is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Anti-Human TGF-α Western Blot Unreduced


Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information.

Country Of Origin: USA

Not for human use.

Research Interest


First Author
Kramer, A
Regulation of daily locomotor activity and sleep by hypothalamic EGF receptor signaling.
Science (New York, N.Y.); 294(5551) pg2511-5
PubMed Id
First Author
Li, Y
Transforming growth factor-alpha: a major human serum factor that promotes human keratinocyte migration.
The Journal of Investigative Dermatology; 126(9) pg2096-105
PubMed Id
First Author
Xian, C J
Specificity of the localization of transforming growth factor-alpha immunoreactivity in colon mucosa.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society; 47(7) pg949-58
PubMed Id