Antigen Affinity Purified Polyclonal Antibodies

Anti-Human TSLP

0 ReviewsSubmit a Review

Product Details

Catalogue Number: 500-P258

Source: Polyclonal Rabbit

Preparation: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hTSLP. Anti-Human TSLP specific antibody was purified by affinity chromatography employing immobilized hTSLP matrix.

Immunogen: E.coli derived Recombinant Human TSLP (PeproTech catalog# 300-62)

Sandwich ELISA: To detect hTSLP by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech’s Biotinylated Anti-Human TSLP (500-P258Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hTSLP.

Anti-Human TSLP Sandwich ELISA

Western Blot: To detect hTSLP by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hTSLP is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Anti-Human TSLP Western Blot Reduced Anti-Human TSLP Western Blot Unreduced

Note:

Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information.

crossreactivity:
Country Of Origin: USA

Not for human use.

Research Interest

product.subtitle.recentcitations

First Author
Hedl, M
Title
STAT3 and STAT5 Signaling Thresholds Determine Distinct Regulation for Innate Receptor-Induced Inflammatory Cytokines, and
Citation
Journal of Immunology; 203(12) pg3325-3338
PubMed Id