Antigen Affinity Purified Polyclonal Antibodies

Anti-Human Wnt-1

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Product Details

Catalogue Number: 500-P250
Anti-Human Wnt-1

Source: Polyclonal Rabbit

Preparation: Produced from sera of rabbits immunized with highly pure recombinant Human Wnt-1. Anti-Human Wnt-1-specific antibody was purified by affinity chromatography employing immobilized Human Wnt-1 matrix.

Immunogen: E.coli derived Recombinant Human Wnt-1 (PeproTech catalog# 120-17)

Sandwich ELISA: To detect hWnt-1 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech’s Biotinylated Anti-Human Wnt-1 (500-P250Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hWnt-1.

Anti-Human Wnt-1 Sandwich ELISA

Western Blot: To detect hWnt-1 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hWnt-1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Anti-Human Wnt-1 Western Blot Reduced Anti-Human Wnt-1 Western Blot Unreduced


Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information.

Country Of Origin: USA

Not for human use.

Research Interest