Antigen Affinity Purified Polyclonal Antibodies

Anti-Murine GM-CSF

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Product Details

Catalogue Number: 500-P65

Source: Polyclonal Rabbit

Preparation: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mGM-CSF. Anti-Murine GM-CSF specific antibody was purified by affinity chromatography employing immobilized mGM-CSF matrix.

Immunogen: E.coli derived Recombinant Murine GM-CSF (PeproTech catalog# 315-03)

Sandwich ELISA: To detect mGM-CSF by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech’s Biotinylated Anti-Murine GM-CSF (500-P65Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mGM-CSF.

Anti-Murine GM-CSF Sandwich ELISA

Western Blot: To detect mGM-CSF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mGM-CSF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Anti-Murine GM-CSF Western Blot Reduced Anti-Murine GM-CSF Western Blot Unreduced


Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information.

Country Of Origin: USA

Not for human use.

Research Interest


First Author
Metcalf, D
Inactivation of PU.1 in adult mice leads to the development of myeloid leukemia.
Proceedings of the National Academy of Sciences of the United States of America; 103(5) pg1486-91
PubMed Id
First Author
Kim, J
Intratumorally Establishing Type 2 Innate Lymphoid Cells Blocks Tumor Growth.
Journal of immunology (Baltimore, Md. : 1950); 196(5) pg2410-23
PubMed Id
First Author
Lentsch, A B
STAT4 and STAT6 regulate systemic inflammation and protect against lethal endotoxemia.
The Journal of Clinical Investigation; 108(10) pg1475-82
PubMed Id