Antigen Affinity Purified Polyclonal Antibodies

Anti-Murine IFN-γ

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Product Details

Catalogue Number: 500-P119

Source: Polyclonal Rabbit

Preparation: Produced from sera of rabbits pre-immunized with highly pure recombinant Murine IFN-γ. Anti-Murine IFN-γ specific antibody was purified by affinity chromatography employing immobilized Murine IFN-γ matrix.

Immunogen: E.coli derived Recombinant Murine IFN-γ (PeproTech catalog# 315-05)

Sandwich ELISA: To detect Murine IFN-γ by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech’s Biotinylated Anti-Murine IFN-γ (500-P119Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Murine IFN-γ.

Anti-Murine IFN-γ Sandwich ELISA

Western Blot: To detect Murine IFN-γ by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Murine IFN-γ is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Anti-Murine IFN-γ Western Blot Reduced Anti-Murine IFN-γ Western Blot Unreduced

Note:

Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information.

crossreactivity:
Country Of Origin: USA

Not for human use.

Research Interest

product.subtitle.recentcitations

First Author
Okoye, I S
Title
Transcriptomics identified a critical role for Th2 cell-intrinsic miR-155 in mediating allergy and antihelminth immunity.
Citation
Proceedings of the National Academy of Sciences of the United States of America; 111(30) pgE3081-90
PubMed Id
First Author
Klooster, J P
Title
Enterocytes, fibroblasts and myeloid cells synergize in anti-bacterial and anti-viral pathways with IL22 as the central cytokine
Citation
Communications Biology; 4(1) pg631
PubMed Id
First Author
Maldonado Galdeano, C
Title
Impact of a probiotic fermented milk in the gut ecosystem and in the systemic immunity using a non-severe protein-energy-malnutrition model in mice.
Citation
BMC Gastroenterology; 11 pg64
PubMed Id