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Antigen Affinity Purified Polyclonal Antibodies

Anti-Murine IL-1α

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Product Details

Catalogue Number: 500-P51A
Anti-Murine IL-1α

Source: Polyclonal Rabbit

Preparation: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mIL-1α. Anti-Murine IL-1α specific antibody was purified by affinity chromatography employing immobilized mIL-1α matrix.

Immunogen: E.coli derived Recombinant Murine IL-1α (PeproTech catalog# 211-11A)

Sandwich ELISA: To detect mIL-1α by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech’s Biotinylated Anti-Murine IL-1α (500-P51ABt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mIL-1α.

Anti-Murine IL-1α Sandwich ELISA

Western Blot: To detect mIL-1α by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-1α is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Anti-Murine IL-1α Western Blot Reduced Anti-Murine IL-1α Western Blot Unreduced


Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information.

Country Of Origin: USA

Not for human use.

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