Antigen Affinity Purified Polyclonal Antibodies

Anti-Murine IL-2

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Product Details

Catalogue Number: 500-P111

Source: Polyclonal Rabbit

Preparation: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mIL-2. Anti-Murine IL-2 specific antibody was purified by affinity chromatography employing immobilized mIL-2 matrix.

Immunogen: E.coli derived Recombinant Murine IL-2 (PeproTech catalog# 212-12)

Sandwich ELISA: To detect mIL-2 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech’s Biotinylated Anti-Murine IL-2 (500-P111Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mIL-2.

Anti-Murine IL-2 Sandwich ELISA

Western Blot: To detect mIL-2 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-2 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Anti-Murine IL-2 Western Blot Reduced Anti-Murine IL-2 Western Blot Unreduced

Note:

Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information.

crossreactivity:
Country Of Origin: USA

Not for human use.

Research Interest

product.subtitle.recentcitations

First Author
Vinderola, G
Title
Milk fermentation products of L. helveticus R389 activate calcineurin as a signal to promote gut mucosal immunity.
Citation
BMC Immunology; 8 pg19
PubMed Id
First Author
Polesso, F
Title
Constitutive expression of NF-κB inducing kinase in regulatory T cells impairs suppressive function and promotes instability and pro-inflammatory cytokine production.
Citation
Scientific Reports; 7(1) pg14779
PubMed Id
First Author
Pérol, L
Title
Loss of immune tolerance to IL-2 in type 1 diabetes.
Citation
Nature Communications; 7 pg13027
PubMed Id