Antigen Affinity Purified Polyclonal Antibodies

Anti-Murine IL-21

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Product Details

Catalogue Number: 500-P278

Source: Polyclonal Rabbit

Preparation: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mIL-21 (Murine Interleukin-21). Anti-Murine IL-21 specific antibody was purified by affinity chromatography employing immobilized mIL-21 matrix.

Immunogen: E.coli derived Recombinant Murine IL-21 (PeproTech catalog# 210-21)

Sandwich ELISA: To detect mIL-21 by sandwich ELISA (using 100μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech’s Biotinylated Anti-Murine IL-21 (500-P278Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mIL-21.

Anti-Murine IL-21 Sandwich ELISA

Western Blot: To detect mIL-21 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-21 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Anti-Murine IL-21 Western Blot Reduced Anti-Murine IL-21 Western Blot Unreduced

Note:

Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information.

crossreactivity:
Country Of Origin: USA

Not for human use.

Research Interest

product.subtitle.recentcitations

First Author
Gu, Y
Title
Forced co-expression of IL-21 and IL-7 in whole-cell cancer vaccines promotes antitumor immunity.
Citation
Scientific Reports; 6 pg32351
PubMed Id