Registration for is no longer available. To order your PeproTech products online, register or logon to

Antigen Affinity Purified Polyclonal Antibodies

Anti-Murine Leptin

0 ReviewsSubmit a Review

Product Details

Catalogue Number: 500-P68
Anti-Murine Leptin

Source: Polyclonal Rabbit

Preparation: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mLeptin. Anti-Murine Leptin specific antibody was purified by affinity chromatography employing immobilized mLeptin matrix.

Immunogen: E.coli derived Recombinant Murine Leptin (PeproTech catalog# 450-31)

Sandwich ELISA: To detect mLeptin by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech’s Biotinylated Anti-Murine Leptin (500-P68Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mLeptin.

Anti-Murine Leptin Sandwich ELISA

Western Blot: To detect mLeptin by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mLeptin is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Anti-Murine Leptin Western Blot Reduced	Anti-Murine Leptin Western Blot Unreduced


Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information.

Country Of Origin: USA

Not for human use.

Research Interest


First Author
Harrison, L
Fluorescent blood-brain barrier tracing shows intact leptin transport in obese mice.
International journal of obesity (2005); 43(6) pg1305-1318
PubMed Id
First Author
Ronkainen, J
Fat mass- and obesity-associated gene Fto affects the dietary response in mouse white adipose tissue.
Scientific Reports; 5 pg9233
PubMed Id