Antigen Affinity Purified Polyclonal Antibodies

Anti-Murine LIX (CXCL6)

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Product Details

Catalogue Number: 500-P146

Source: Polyclonal Rabbit

Preparation: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mLIX. Anti-Murine LIX specific antibody was purified by affinity chromatography employing immobilized mLIX matrix.

Immunogen: E.coli derived Recombinant Murine LIX (CXCL6) (92 a.a.) (PeproTech catalog# 250-17)

Sandwich ELISA: To detect mLIX by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech’s Biotinylated Anti-Murine LIX (500-P146Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mLIX.

Anti-Murine LIX (CXCL6) Sandwich ELISA

Western Blot: To detect mLIX by Western Blot analysis this antibody can be used at a concentration of 0.1- 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mLIX is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Anti-Murine LIX (CXCL6) Western Blot Reduced Anti-Murine LIX (CXCL6) Western Blot Unreduced

Note:

Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information.

crossreactivity:
Country Of Origin: USA

Not for human use.

Research Interest

product.subtitle.recentcitations

First Author
Chandrasekar, B
Title
Ischemia-reperfusion of rat myocardium activates nuclear factor-KappaB and induces neutrophil infiltration via lipopolysaccharide-induced CXC chemokine.
Citation
Circulation; 103(18) pg2296-302
PubMed Id
First Author
Speetjens, F M
Title
Disrupted expression of CXCL5 in colorectal cancer is associated with rapid tumor formation in rats and poor prognosis in patients.
Citation
Clinical cancer research : an official journal of the American Association for Cancer Research; 14(8) pg2276-84
PubMed Id
First Author
Van Lint, P
Title
Resistance of collagenase-2 (matrix metalloproteinase-8)-deficient mice to TNF-induced lethal hepatitis.
Citation
Journal of immunology (Baltimore, Md. : 1950); 175(11) pg7642-9
PubMed Id