Antigen Affinity Purified Polyclonal Antibodies

Anti-Murine TNF-α (Polyclonal Rabbit)

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Product Details

Catalogue Number: 500-P64
Description:
Anti-Murine TNF-α (Polyclonal Rabbit)
 

Source: Polyclonal Rabbit

Preparation: Produced from sera of rabbits pre-immunized with highly pure recombinant Murine TNF-α. Anti-Murine TNF-α specific antibody was purified by affinity chromatography employing immobilized Murine TNF-α matrix.

Immunogen: E.coli derived Recombinant Murine TNF-α (PeproTech catalog# 315-01A)

Sandwich ELISA: To detect Murine TNF-α by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech’s Biotinylated Anti-Murine TNF-α (500-P64Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Murine TNF-α.

Anti-Murine TNF-α Sandwich ELISA

Western Blot: To detect Murine TNF-α by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Murine TNF-α is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Anti-Murine TNF-α Western Blot Reduced Anti-Murine TNF-α Western Blot Unreduced

Note:

Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information.

crossreactivity:
Country Of Origin: USA

Not for human use.

Research Interest

product.subtitle.recentcitations

First Author
Klooster, J P
Title
Enterocytes, fibroblasts and myeloid cells synergize in anti-bacterial and anti-viral pathways with IL22 as the central cytokine
Citation
Communications Biology; 4(1) pg631
PubMed Id
First Author
Tayabali, A F
Title
Comparison of the virulence potential of Acinetobacter strains from clinical and environmental sources.
Citation
PLoS ONE; 7(5) pge37024
PubMed Id
First Author
Maldonado Galdeano, C
Title
Impact of a probiotic fermented milk in the gut ecosystem and in the systemic immunity using a non-severe protein-energy-malnutrition model in mice.
Citation
BMC Gastroenterology; 11 pg64
PubMed Id