Antigen Affinity Purified Polyclonal Antibodies

Anti-Rat IL-10

0 ReviewsSubmit a Review

Product Details

Catalogue Number: 500-P139

Source: Polyclonal Rabbit

Preparation: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant Rat IL-10. Anti-Rat IL-10 specific antibody was purified by affinity chromatography employing immobilized Rat IL-10 matrix.

Immunogen: E.coli derived Recombinant Rat IL-10 (PeproTech catalog# 400-19)

Sandwich ELISA: To detect Rat IL-10 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech’s Biotinylated Anti-Rat IL-10 (500-P139Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat IL-10.

Anti-Rat IL-10 Sandwich ELISA

Western Blot: To detect Rat IL-10 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat IL-10 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Anti-Rat IL-10 Western Blot Reduced Anti-Rat IL-10 Western Blot Unreduced

Note:

Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information.

crossreactivity:
Rat
Country Of Origin: USA

Not for human use.

Research Interest

product.subtitle.recentcitations

First Author
Curato, C
Title
Identification of noncytotoxic and IL-10-producing CD8+AT2R+ T cell population in response to ischemic heart injury.
Citation
Journal of immunology (Baltimore, Md. : 1950); 185(10) pg6286-93
PubMed Id
First Author
Schif-Zuck, S
Title
Coadministration of plasmid DNA constructs encoding an encephalitogenic determinant and IL-10 elicits regulatory T cell-mediated protective immunity in the central nervous system.
Citation
Journal of immunology (Baltimore, Md. : 1950); 177(11) pg8241-7
PubMed Id
First Author
Foright, R M
Title
Compensatory eating behaviors in male and female rats in response to exercise training.
Citation
American journal of physiology. Regulatory, integrative and comparative physiology; 319(2) pgR171-R183
PubMed Id