Antigen Affinity Purified Polyclonal Antibodies

Anti-Rat VEGF165

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Product Details

Catalogue Number: 500-P275

Source: Polyclonal Rabbit

Preparation: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant Rat VEGF (Rat Vascular Endothelial Growth Factor). Anti-Rat VEGF specific antibody was purified by affinity chromatography employing immobilized Rat VEGF matrix.

Immunogen: E.coli derived Recombinant Rat VEGF165 (PeproTech catalog# 400-31)

Sandwich ELISA: To detect Rat VEGF by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech’s Biotinylated Anti-Rat VEGF (500-P275Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat VEGF.

Anti-Rat VEGF 165 Sandwich ELISA

Western Blot: To detect Rat VEGF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat VEGF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Anti-Rat VEGF 165 Western Blot Reduced Anti-Rat VEGF 165 Western Blot Unreduced

Note:

Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information.

crossreactivity:
Rat
Country Of Origin: USA

Not for human use.

Research Interest

product.subtitle.recentcitations

First Author
Genetos, D C
Title
Oxygen tension modulates neurite outgrowth in PC12 cells through a mechanism involving HIF and VEGF.
Citation
Journal of molecular neuroscience : MN; 40(3) pg360-6
PubMed Id