Antigen Affinity Purified Polyclonal Antibodies

Source: Polyclonal Rabbit

Preparation: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant Rat VEGF (Rat Vascular Endothelial Growth Factor). Anti-Rat VEGF specific antibody was purified by affinity chromatography employing immobilized Rat VEGF matrix.

Immunogen: E.coli derived Recombinant Rat VEGF₁₆₅ (PeproTech catalog# 400-31)

Sandwich ELISA: To detect Rat VEGF by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech’s Biotinylated Anti-Rat VEGF (500-P275Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat VEGF.

Western Blot: To detect Rat VEGF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat VEGF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Note:

Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information.