Biotinylated Polyclonal Antibodies

Source: Polyclonal Rabbit

Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human FGF-23. Anti-Human FGF-23-specific antibody was purified by affinity chromatography and then biotinylated.

Immunogen: E.coli-derived, 25.5kDa Recombinant Human FGF-23 (PeproTech Catalog# 100-52)

Sandwich ELISA: To detect Human FGF-23 by sandwich ELISA (using 100ul/well), a concentration of 0.25-1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech’s Polyclonal Anti-Human FGF-23 (500-P319) as a capture antibody, allows the detection of at least 2000-4000 pg/ml of Recombinant Human FGF-23.

Western Blot: To detect Human FGF-23 by Western Blot analysis, this antibody can be used at a concentration of 0.1-0.2ug/ml. When used in conjunction with compatible development reagents, the detection limit for Recombinant Human FGF-23 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Note:

1mg will be provided as 2x500μg.