Biotinylated Polyclonal Antibodies

Source: Polyclonal Rabbit

Preparation: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hMIA-2 (human MIA-2). Anti-Human MIA-2 specific antibody was purified by affinity chromatography and then biotinylated.

Immunogen: E.coli derived Recombinant Human MIA-2 (PeproTech catalog# 130-02)

Sandwich ELISA: To detect Human MIA-2 by sandwich ELISA (using 100 ml/well) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech’s Polyclonal Anti- Human MIA-2 (500-P255) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant Human MIA-2.

Western Blot: To detect Human MIA-2 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 mg/ml. When used in conjunction with compatible development reagents the detection limit for recombinant Human MIA-2 is 1.5 – 3.0 ng/lane, under either reducing or non-reducing conditions. 

Note:

1mg will be provided as 2x500μg.