Biotinylated Polyclonal Antibodies

Source: Polyclonal Rabbit

Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human MMP-3. Anti-Human MMP-3-specific antibody was purified by affinity chromatography and then biotinylated.

Immunogen: E.coli derived Recombinant Human MMP-3 (PeproTech catalog# 420-03)

Sandwich ELISA: To detect Human MMP-3 by sandwich ELISA (using 100μl/well), a concentration of 0.25-1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech’s Polyclonal Anti-Human MMP-3 (500-P324) as a capture antibody, allows the detection of at least 2000-4000 pg/ml of Recombinant Human MMP-3.

Western Blot: To detect Human MMP-3 by Western Blot analysis, this antibody can be used at a concentration of 0.1-0.2 μg/ml. When used in conjunction with compatible development reagents, the detection limit for Recombinant Human MMP-3 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Note:

1mg will be provided as 2x500μg.