Biotinylated Polyclonal Antibodies

Source: Polyclonal Rabbit

Preparation: Produced from sera of rabbits immunized with highly pure recombinant Rat SDF-1α. Anti-Rat SDF-1α-specific antibody was purified by affinity chromatography and then biotinylated.

Immunogen: E.coli-derived, 7.9 kDa Recombinant Rat SDF-1α (PeproTech catalog# 400-32A)

Sandwich ELISA: To detect Rat SDF-1α by sandwich ELISA (using 100 μl/well), a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech’s Polyclonal Anti-Rat SDF-1α (500-P315) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant Rat SDF-1α.

Western Blot: To detect Rat SDF-1α by Western Blot analysis, this antibody can be used at a concentration of 0.1 - 0.2 μg/ml. When used in conjunction with compatible development reagents, the detection limit for recombinant Rat SDF-1α is 1.5 – 3.0 ng/lane, under either reducing or non-reducing conditions.

Note:

1mg will be provided as 2x500μg.