PeproTech’s Cell Passaging/Non-Enzymatic Detachment Buffer is a companion product for PeproTech’s Stem Cell Media. This reagent contains PBS, HEPES, and EDTA, and is capable of detaching hESCs and hiPSCs from cell plates/dishes during cell passing steps. It is recommended for non-enzymatic passaging protocols, and to be used in conjunction with PeproTech’s Animal-Free Human Vitronectin Matrix as a cell culture surface-coating reagent.
Note: On December 1, 2022 this product will be discontinued. Gibco™ Cell Dissociation Buffer, enzyme-free, PBS (Catalog# 13151014) and Invitrogen™ Accutase - Enzyme Cell Detachment Medium (Catalog# 00-4555-56) can be used as a substitution for this product.
Not for human use.