PeproGrow™ hMSC (Mesenchymal Stem Cell) Media

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Product Details

Catalogue Number: XF-HMSC-500

PeproGrow hMSC (Mesenchymal Stem Cell) Medium is a xeno-free, human serum containing, phenol red-free complete media formulation originally designed for the in vitro expansion of adipose-derived human mesenchymal stem cells (ADMSCs) while maintaining full multipotency. Since its design, this media formulation has been shown to be suitable for the sustained growth of adipose tissue-derived, bone marrow-derived, umbilical cord-derived, placental-derived, and urine-derived MSCs in both adherent and suspension culture. For optimal results, culturing should be conducted on a surface coated with PeproTech’s Animal-Free Human Vitronectin Matrix as a surface-coating reagent; however, other suitable extracellular matrix (ECM) proteins, such as fibronectin or vitronectin, can be used. PeproGrow hMSC Medium was designed and developed in collaboration with American CryoStem Corporation, and is supplied as a 500mL bottle of PeproGrow hMSC Basal Medium containing a human serum component, and a separate, lyophilized vial of animal-free PeproGrow hMSC Growth Factor Supplement. The addition of the separate, lyophilized growth factor supplement to the basal medium results in a complete medium containing all growth factors and supplements necessary for optimal expansion of human mesenchymal stem cells in culture. Additional companion products, including PeproTech’s Animal-Free Vitronectin Matrix and Buffer Kit, are available separately.

Note: On December 1, 2022 this product will be discontinued. Gibco™ StemPro™ MSC SFM XenoFree (Catalog# A1067501) can be used as a substitution for this product.


MSC growth curve


Note: The manual for this media can be found here

Country Of Origin: USA

Not for human use.

Research Interest


First Author
Mishra, P
Fluorescence Imaging of Actin Turnover Parses Early Stem Cell Lineage Divergence and Senescence.
Scientific Reports; 9(1) pg10377
PubMed Id