Animal-Free Recombinant Human Flt3-Ligand 0 ReviewsSubmit a Review Product Details Catalogue Number: AF-300-19 Description: Flt3-Ligand is a growth factor that regulates proliferation of early hematopoietic cells. Flt3-Ligand binds to cells expressing the tyrosine kinase receptor Flt3. Flt3-Ligand, by itself does not stimulate proliferation of early hematopoietic cells, but synergizes with other CSFs and interleukins to induce growth and differentiation. Unlike SCF, Flt3-Ligand exerts no activity on mast cells. Multiple isoforms of Flt3-Ligand have been identified. The predominant biologically active form is anchored to the cell surface as the extracellular domain of a transmembrane protein (209 a.a.). The membrane-bound isoform can be proteolytically cleaved to generate a biologically active soluble isoform. Recombinant Human Flt3-Ligand is a soluble 17.6 kDa protein consisting of 155 amino acid residues. Manufactured using all Animal-Free reagents. Source: E.coli Synonyms: Fms-related tyrosine kinase 3 ligand, Flt3L AA Sequence: TQDCSFQHSP ISSDFAVKIR ELSDYLLQDY PVTVASNLQD EELCGGLWRL VLAQRWMERL KTVAGSKMQG LLERVNTEIH FVTKCAFQPP PSCLRFVQTN ISRLLQETSE QLVALKPWIT RQNFSRCLEL QCQPDSSTLP PPWSPRPLEA TAPTA Purity: ≥ 98% by SDS-PAGE gel and HPLC analyses. Biological Activity: The ED50 as determined by the dose-dependent stimulation of the proliferation of human AML5 cells is ≤ 1.0 ng/ml, corresponding to a specific activity of ≥ 1 x 106 units/mg. This product has been tested against the WHO standard. International Unit information can be found here. Calculated Molecular Weight: 17.6 kDa Accession Number: P49771 Gene ID: 2322 Endotoxin: Endotoxin level is < 0.01 ng/ug of protein (< 0.1 EU/ug) crossreactivity: Human, Human + Hamster, Monkey, Mouse, Rabbit References PubMed SDS CoA Search Product Line Country Of Origin: USA Not for human use. Research Interest Cancer Cell Culture Immune System Stem Cells & Differentiation product.subtitle.recentcitations First Author McGrath, E Title Targeting specificity of APOBEC-based cytosine base editor in human iPSCs determined by whole genome sequencing. Citation Nature Communications; 10(1) pg5353 PubMed Id 31767844 First Author Xu, Y Title TAF1 plays a critical role in AML1-ETO driven leukemogenesis. Citation Nature Communications; 10(1) pg4925 PubMed Id 31664040 First Author Song, L Title Functionalization of Brain Region-specific Spheroids with Isogenic Microglia-like Cells. Citation Scientific Reports; 9(1) pg11055 PubMed Id 31363137
