Neurotrophins

Recombinant Human GDNF

Product Details

Catalogue Number: 450-10
Description:

GDNF is a disulfide-linked, homodimeric neurotrophic factor structurally related to Artemin, Neurturin and Persephin.  These proteins belong to the cysteine-knot superfamily of growth factors that assume stable dimeric protein structures.  GDNF signals through a multicomponent receptor system, composed of a RET and one of the four GFRα (α1-α4) receptors.  GDNF specifically promotes dopamine uptake and survival, and morphological differentiation of midbrain neurons.  Using a Parkinson’s disease mouse model, GDNF has been shown to improve conditions such as bradykinesia, rigidity, and postural instability.  The functional human GDNF ligand is a disulfide-linked homodimer consisting of two 15 kDa polypeptide chains called monomers.  Each monomer contains seven conserved cysteine residues, including Cys-101, which is used for inter-chain disulfide bridging, and others that are involved in the intramolecular ring formation known as the cysteine-knot configuration. The calculated molecular weight of Recombinant Human GDNF is 30.4 kDa.

Source: E.coli

Synonyms: Glial-Derived Neurotrophic Factor, ATF-1

AA Sequence (monomer): MSPDKQMAVL PRRERNRQAA AANPENSRGK GRRGQRGKNR GCVLTAIHLN VTDLGLGYET KEELIFRYCS GSCDAAETTY DKILKNLSRN RRLVSDKVGQ ACCRPIAFDD DLSFLDDNLV YHILRKHSAK RCGCI

Purity: ≥ 98% by SDS-PAGE gel and HPLC analyses.

Biological Activity: Determined by a cell proliferation assay using SH-SY5Y cells. The expected ED50 for this effect is 1.0-10.0 ng/ml.

This product has been tested against the WHO standard. International Unit information can be found here.

Calculated Molecular Weight: 30.4 kDa

Accession Number: P39905

Gene ID: 2668

crossreactivity:
Country Of Origin: USA

Not for human use.

Research Interest

product.subtitle.recentcitations

First Author
Hanson, M G
Title
Cyclic AMP elevation is sufficient to promote the survival of spinal motor neurons in vitro.
Citation
The Journal of neuroscience : the official journal of the Society for Neuroscience; 18(18) pg7361-71
PubMed Id
First Author
Liou, J C
Title
Regulation of quantal secretion by neurotrophic factors at developing motoneurons in Xenopus cell cultures.
Citation
The Journal of Physiology; 503 ( Pt 1) pg129-39
PubMed Id
First Author
Li, J
Title
An integrated multi-omic analysis of iPSC-derived motor neurons from C9ORF72 ALS patients
Citation
Iscience; 24(11):103221
PubMed Id

product.subtitle.productreviews

Existing reviews
High quality product
Used for human stem cells. Awesome product!
From:  Danielle | Date:  26/08/2020
Cortical Neuron Differentiation
Very consistent for culturing human stem cells
From:  Danielle | Date:  24/08/2021
iPSC-derived neurons
Used successfully as part of a battery of growth factors and small molecules to differentiate iPSCs into neurons.
From:  Mandi | Date:  25/08/2021